Ka Hoʻololi ʻana o ka Mycobacterium Tuberculosis INH
Inoa huahana
Pahu ʻIke Hoʻololi INH Mycobacterium Tuberculosis HWTS-RT137 (Kīkoʻo Melting)
ʻAlapia
ʻO Mycobacterium tuberculosis, ʻo Tubercle bacillus (TB) pōkole, ʻo ia ka bacteria pathogenic e hoʻoulu ai i ka maʻi tuberculosis. I kēia manawa, ʻo nā lāʻau lapaʻau anti-tuberculosis laina mua i hoʻohana pinepine ʻia e komo pū me INH, rifampicin a me hexambutol, a pēlā aku. ʻO nā lāʻau lapaʻau anti-tuberculosis laina lua e komo pū ana me fluoroquinolones, amikacin a me kanamycin, a pēlā aku. ʻO nā lāʻau lapaʻau hou i hoʻomohala ʻia he linezolid, bedaquiline a me delamani, a pēlā aku. Eia naʻe, ma muli o ka hoʻohana hewa ʻana o nā lāʻau anti-tuberculosis a me nā ʻano o ke ʻano o ka paia cell o mycobacterium tuberculosis, hoʻomohala ka mycobacterium tuberculosis i ke kūʻē ʻana i ka lāʻau i nā lāʻau anti-tuberculosis, kahi e lawe mai ai i nā pilikia koʻikoʻi i ka pale ʻana a me ka mālama ʻana i ka maʻi tuberculosis.
Kanal
| ʻOhana | Waikawa nucleic MP |
| ROX | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤-18℃ |
| Ola papa | 12 mahina |
| ʻAno Laʻana | huʻa |
| CV | ≤5% |
| LoD | ʻO ka palena ʻike no nā bacteria INH ʻano hihiu he 2x103 bacteria/mL, a ʻo ka palena ʻike no nā bacteria mutant he 2x103 bacteria/mL. |
| Kūlana kikoʻī | a. ʻAʻohe hopena kea ma waena o ka genome kanaka, nā mycobacteria ʻē aʻe ʻaʻole tuberculous a me nā pathogens pneumonia i ʻike ʻia e kēia pahu.b. Ua ʻike ʻia nā wahi mutation o nā genes ʻē aʻe i kūʻē i ka lāʻau lapaʻau ma ka Mycobacterium tuberculosis ʻano hihiu, e like me ka ʻāpana hoʻoholo kūʻē o ka gene rifampicin rpoB, a ʻaʻole i hōʻike nā hopena hoʻāʻo i ke kūʻē ʻana i ka INH, e hōʻike ana ʻaʻohe cross reactivity. |
| Nā Mea Hana Pili | Nā ʻōnaehana PCR Manawa Maoli SLAN-96PNā ʻōnaehana PCR Manawa Maoli BioRad CFX96LightCycler480®ʻŌnaehana PCR Manawa Maoli |
Kahe Hana
Inā hoʻohana ʻoe i ka Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (hiki ke hoʻohana ʻia me ka Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. no ka unuhi ʻana, e hoʻohui i 200μL o ka mana maikaʻi ʻole a me ka hāpana sputum i hana ʻia e hoʻāʻo ʻia ma ke kaʻina, a e hoʻohui i 10μL o ka mana kūloko i loko o ka mana maikaʻi ʻole, ka hāpana sputum i hana ʻia e hoʻāʻo ʻia, a pono e hoʻokō pono ʻia nā hana ma hope e like me nā kuhikuhi unuhi. ʻO ka nui o ka hāpana i unuhi ʻia he 200μL, a ʻo ka nui o ka elution i ʻōlelo ʻia he 100μL.
