Mycobacterium Tuberculosis INH Mutation
inoa huahana
HWTS-RT137 Mycobacterium Tuberculosis INH Mutation Detection Kit (Melting Curve)
Epidemiology
ʻO ka Mycobacterium tuberculosis, i kapa koke ʻia ʻo Tubercle bacillus (TB), ʻo ia ka maʻi maʻi pathogenic e hoʻoulu ai i ka maʻi maʻi. I kēia manawa, ʻo nā lāʻau anti-tuberculosis laina mua i hoʻohana pinepine ʻia me INH, rifampicin a me hexambutol, a me nā mea ʻē aʻe. Hoʻoulu ka mycobacterium tuberculosis i ka lāʻau kūʻē i nā lāʻau anti-tuberculosis, kahi e lawe mai ai i nā pilikia koʻikoʻi i ka pale ʻana a me ka mālama ʻana i ka maʻi maʻi.
Kanal
| FAM | ʻakika nucleic MP |
| ROX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | ʻūhā |
| CV | ≤5% |
| LoD | ʻO ka palena ʻike no ka bacteria INH ʻano hihiu he 2x103 bacteria/mL, a ʻo ka palena ʻike no ka bacteria mutant he 2x103 bacteria/mL. |
| kikoo | a. ʻAʻohe hopena keʻa ma waena o ka genome kanaka, nā mycobacteria nontuberculous ʻē aʻe a me nā maʻi maʻi pneumonia i ʻike ʻia e kēia kit.b. Ua ʻike ʻia nā pae hoʻololi o nā genes kū'ē lāʻau ʻē aʻe i loko o ka Mycobacterium tuberculosis ʻano hihiu, e like me ke kūʻē ʻana i ka ʻāpana o ka rifampicin rpoB gene, a ua hōʻike ʻia nā hopena hoʻāʻo ʻaʻole kūʻē i ka INH, e hōʻike ana ʻaʻole hoʻololi kea. |
| Nā mea hana pili | SLAN-96P Pūnaehana PCR Manawa maoliBioRad CFX96 Pūnaehana PCR Manawa maoliLightCycler480®Pūnaehana PCR manawa maoli |
Holo Hana
Inā hoʻohana i ka Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) e Jiangsu Macro & Micro-Test Med-Tech Co., no ka unuhi ʻana i ke kaʻina hana ʻino a me ka hoʻohui ʻana i ka 20μL. kaʻina, a hoʻohui i ka 10μL o ka mana kūloko i loko o ka mana maikaʻi ʻole, hana ʻia ka sputum sample e hoʻāʻo ʻia, a pono e hoʻokō pono ʻia nā ʻanuʻu ma hope e like me nā ʻōlelo aʻoaʻo. ʻO 200μL ka nui o ka laʻana i unuhi ʻia, a ʻo ka nui elution i ʻōlelo ʻia he 100μL.
