Mycobacterium Tuberculosis Isoniazid Resistance Mutation

ʻO ka wehewehe pōkole:

He kūpono kēia kit no ka ʻike qualitative o nā pae hoʻololi nui i nā laʻana sputum kanaka i hōʻiliʻili ʻia mai nā maʻi maʻi maʻi Tubercle bacillus e alakaʻi i ka mycobacterium tuberculosis isoniazid resistance: InhA promoter region -15C>T, -8T>A, -8T>C;ʻĀpana hoʻolaha AhpC -12C>T, -6G>A;homozygous mutation o KatG 315 codon 315G>A, 315G>C .

E hoʻouna i ka leka uila iā mākou

Huahana Huahana

Inoa mea kūʻai

HWTS-RT137 Mycobacterium Tuberculosis Isoniazid Resistance Mutation Detection Kit (Melting Curve)

Epidemiology

ʻO ka Mycobacterium tuberculosis, i kapa koke ʻia ʻo Tubercle bacillus (TB), ʻo ia ka maʻi maʻi pathogenic e hoʻoulu ai i ka maʻi maʻi.I kēia manawa, ʻo nā lāʻau anti-tuberculosis laina mua i hoʻohana pinepine ʻia me isoniazid, rifampicin a me hexambutol, a me nā mea ʻē aʻe. Eia nō naʻe, ma muli o ka hoʻohana hewa ʻana i nā lāʻau anti-tuberculosis a me nā ʻano o ke ʻano o ka pā hale o ka mycobacterium tuberculosis, ua hoʻomohala ka mycobacterium tuberculosis i ka lāʻau kūʻē i nā lāʻau anti-tuberculosis, kahi e lawe mai ai i nā pilikia koʻikoʻi i ka pale ʻana a me ka mālama ʻana i ka maʻi maʻi.

Kanal

FAM	ʻakika nucleic MP
ROX	Hoomalu Kuloko

Nā ʻāpana ʻenehana

Waihona	≤-18 ℃
Kau-ola	12 mahina
ʻAno Hoʻohālike	ʻūhā
CV	≤5%
LoD	He 2x103 bacteria/mL ka palena ʻike no ka bacteria kūʻē isoniazid ʻano hihiu, a ʻo ka palena ʻike no ka bacteria mutant he 2x103 bacteria/mL.
kikoo	a.ʻAʻohe hopena keʻa ma waena o ka genome kanaka, nā mycobacteria nontuberculous ʻē aʻe a me nā maʻi maʻi pneumonia i ʻike ʻia e kēia kit.b.Ua ʻike ʻia nā pae hoʻololi o nā genes kūpaʻa lāʻau ʻē aʻe i loko o ka Mycobacterium tuberculosis ʻano hihiu, e like me ke kūʻē ʻana i ka ʻāpana o ka rifampicin rpoB gene, a ua hōʻike ʻia nā hopena hoʻāʻo ʻaʻole kūʻē i ka isoniazid, e hōʻike ana ʻaʻole hoʻololi kea.
Nā mea hana pili	SLAN-96P Pūnaehana PCR Manawa maoliBioRad CFX96 Pūnaehana PCR Manawa maoli LightCycler480® Pūnaehana PCR manawa maoli

Holo Hana

Inā hoʻohana i ka Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. no ka unuhi ʻana, hoʻohui i 200μL o ka mana kino a me ka ho'āʻo sputum sample e ho'āʻo ma ke kaʻina, a hoʻohui i 10μL o ka mana o loko o ka hoʻokaʻawale ʻana i loko o ka mana maikaʻi ʻole, hoʻāʻo ʻia ka sputum sample e hoʻāʻo ʻia, a pono e hoʻokō pono ʻia nā ʻanuʻu ma hope e like me nā ʻōlelo aʻoaʻo.He 200 ka nui o ka laana i unuhiiaμL, a ʻo ka nui o ka elution i ʻōlelo ʻia he 100μL.