Mycoplasma Pneumoniae （MP）
inoa huahana
HWTS-RT024 Mycoplasma Pneumoniae（MP） Puke ʻImi Nucleic Acid (Fluorescence PCR)
Epidemiology
ʻO ka Mycoplasma pneumoniae (MP) kekahi ʻano o ka microorganism prokaryotic liʻiliʻi loa, aia ma waena o ka maʻi bacteria a me ka maʻi maʻi, me ke ʻano o ke kelepona akā ʻaʻohe paia o ke kelepona. ʻO MP ke kumu nui o ka maʻi hanu kanaka, ʻoi aku i nā keiki a me nā ʻōpio. Hiki iā ia ke hoʻoulu i ka maʻi maʻi mycoplasma kanaka, ka maʻi ʻōpū o nā keiki a me ka maʻi pneumonia atypical. He ʻokoʻa nā hōʻike lapaʻau, ʻo ka hapa nui o ia mau mea he ʻuʻu nui, ke kuni, ke anu, ke poʻo, ka ʻeha ʻeha. ʻO ka maʻi maʻi o ka ʻōpū o luna a me ka bronchial pneumonia ka mea maʻamau. Hiki i kekahi poʻe maʻi ke hoʻomohala mai ka maʻi ʻōpū o luna a hiki i ka maʻi pneumonia koʻikoʻi, hiki mai ka pilikia hanu a me ka make.
Kanal
| FAM | Mycoplasma pneumoniae |
| VIC/HEX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | Sputum、Oropharyngeal swab |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 200 Kope/mL |
| kikoo | a） Ka hoʻololi ʻana o ke keʻa: ʻaʻohe hana keʻa me Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacterium tuberculosis, Legionella pseinosaudoma. Acinetobacter baumannii, ka ma'i Influenza A, ka ma'i Influenza B, ka ma'i ma'i Parainfluenza type I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Respiratory syncytial virus a me kanaka genomic nucleic acid. b）Ka hiki ke pale aku i ka hoʻopilikia: ʻaʻohe mea keakea i ka wā i hoʻāʻo ʻia ai nā mea keakea me kēia mau ʻano: hemoglobin (50mg/L), bilirubin (20mg/dL), mucin (60mg/mL), 10% (v/v) koko kanaka, levofloxacin (10μg/mL), moxifloxacin(0. (80μg/mL), azithromycin (1mg/mL), clarithromycin (125μg/mL), erythromycin (0.5g/L), doxycycline (50mg/L), minocycline (0.1g/L). |
| Nā mea hana pili | Hoʻohana ʻia ʻo Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR manawa maoli (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli (FQD-96A, ʻenehana Hangzhou Bioer) MA-6000 Mea Hoʻokele Thermal Cycler Manawa maoli (Suzhou Molarray Co., Ltd.) BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
(1) Laʻana Sputum
Manaʻo ʻia ka hoʻoneʻe ʻana: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (000HWC Acid Extractor). HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Hoʻohui i 200µL o ka paʻakai maʻamau i ka precipitate i hana ʻia. Pono e hana ʻia ka unuhi ʻana ma hope e like me ke kuhikuhi no ka hoʻohana ʻana. ʻO ka nui o ka elution i manaʻo ʻia ʻo 80µL. Manaʻo ʻia ka hoʻoulu ʻana: Nucleic Acid Extraction a i ʻole Purification Reagent (YDP315-R). Pono e hoʻokō pono ʻia ka unuhi ʻana e like me ke kuhikuhi no ka hoʻohana ʻana. ʻO ka nui o ka elution i manaʻo ʻia he 60µL.
(2) Oropharyngeal swab
Manaʻo ʻia ka hoʻoneʻe ʻana: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (000HWC Acid Extractor). HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Pono e hana ʻia ka unuhi e like me ke kuhikuhi no ka hoʻohana ʻana. ʻO 200µL ka nui o ka unuhi ʻana i manaʻo ʻia, a ʻo ka nui o ka elution i manaʻo ʻia ʻo 80µL. Manaʻo ʻia ka reagent unuhi: QIAamp Viral RNA Mini Kit (52904) a i ʻole Nucleic Acid Extraction a i ʻole Purification Reagent (YDP315-R). Pono e hoʻokō pono ʻia ka unuhi ʻana e like me ke kuhikuhi no ka hoʻohana ʻana. ʻO 140µL ka nui o ka unuhi ʻana i manaʻo ʻia, a ʻo ka nui elution i manaʻo ʻia he 60µL.
