ʻO ka Mycoplasma Pneumoniae (MP)
Inoa huahana
Pahu ʻIke Waikawa Nukleika HWTS-RT024 Mycoplasma Pneumoniae（MP） (Fluorescence PCR)
ʻAlapia
ʻO ka Mycoplasma pneumoniae (MP) kahi ʻano o ka microorganism prokaryotic liʻiliʻi loa, aia ma waena o ka bacteria a me ka virus, me ke ʻano o ke kelepona akā ʻaʻohe paia o ke kelepona. ʻO MP ke kumu nui o ka maʻi hanu kanaka, ʻoi aku hoʻi i nā keiki a me nā ʻōpio. Hiki iā ia ke hana i ka pneumonia mycoplasma kanaka, ka maʻi hanu o nā keiki a me ka pneumonia atypical. He ʻokoʻa nā hōʻike lapaʻau, ʻo ka hapa nui o ia mau mea he ʻū nui, ke kuni, ke anu, ka ʻeha poʻo, ka ʻāʻī ʻeha. ʻO ka maʻi hanu o luna a me ka pneumonia bronchial ka mea maʻamau. Hiki i kekahi mau maʻi ke ulu mai ka maʻi hanu o luna a hiki i ka pneumonia koʻikoʻi, hiki ke loaʻa ka pilikia hanu koʻikoʻi a me ka make.
Kanal
| ʻOhana | ʻO ka maʻi pneumonia Mycoplasma |
| VIC/HEX | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤-18℃ |
| Ola papa | 12 mahina |
| ʻAno Laʻana | ʻUlu, ʻUlu ʻōpū |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 200 Kope/mL |
| Kūlana kikoʻī | a) Ke kū'ē ʻana: ʻaʻohe kū'ē ʻana me Ureaplasma urealyticum, Mycoplasma genitalium, Mycoplasma hominis, Streptococcus pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, Klebsiella pneumoniae, Staphylococcus aureus, Mycobacterium tuberculosis, Legionella pneumophila, Pseudomonas aeruginosa, Acinetobacter baumannii, Influenza A virus, Influenza B virus, Parainfluenza virus type I/II/III/IV, Rhinovirus, Adenovirus, Human metapneumovirus, Respiratory syncytial virus a me ka human genomic nucleic acid. b) Ka hiki ke pale aku i ke komo ʻana: ʻaʻohe komo ʻana i ka wā i hoʻāʻo ʻia ai nā mea komo me kēia mau ʻano: hemoglobin (50mg/L), bilirubin (20mg/dL), mucin (60mg/mL), 10% (v/v) koko kanaka, levofloxacin (10μg/mL), moxifloxacin (0.1g/L), gemifloxacin (80μg/mL), azithromycin (1mg/mL), clarithromycin (125μg/mL), erythromycin (0.5g/L), doxycycline (50mg/L), minocycline (0.1g/L). |
| Nā Mea Hana Pili | ʻŌnaehana PCR Manawa Maoli Biosystems 7500 i hoʻopili ʻia Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 QuantStudio®5 mau ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR Manawa Maoli SLAN-96P (Hongshi Medical Technology Co., Ltd.) LightCycler®ʻŌnaehana PCR Manawa Maoli 480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus (FQD-96A, ʻenehana Hangzhou Bioer) MA-6000 Mea Hoʻopololei Wela Manawa Maoli (Suzhou Molarray Co., Ltd.) ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
(1) Hāpana ʻūpā
Mea hoʻohuihui i manaʻo ʻia: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. E hoʻohui i 200µL o ka saline maʻamau i ka precipitate i hana ʻia. Pono e hana ʻia ka unuhi ʻana ma hope e like me ke kuhikuhi no ka hoʻohana ʻana. ʻO ka nui o ka elution i manaʻo ʻia he 80µL. Mea hoʻohuihui i manaʻo ʻia: Nucleic Acid Extraction a i ʻole Purification Reagent (YDP315-R). Pono e hana pono ʻia ka unuhi ʻana e like me ke kuhikuhi no ka hoʻohana ʻana. ʻO ka nui o ka elution i manaʻo ʻia he 60µL.
(2) ʻUmi ʻōpū
Mea hoʻohuihui i manaʻo ʻia: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-50, HWTS-3017-32, HWTS-3017-48, HWTS-3017-96) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Pono e hana ʻia ka unuhi ʻana e like me ke kuhikuhi no ka hoʻohana ʻana. ʻO ka nui o ka unuhi ʻana i manaʻo ʻia o ka laʻana he 200µL, a ʻo ka nui o ka elution i manaʻo ʻia he 80µL. Mea hoʻohuihui i manaʻo ʻia: QIAamp Viral RNA Mini Kit (52904) a i ʻole Nucleic Acid Extraction a i ʻole Purification Reagent (YDP315-R). Pono e hana pono ʻia ka unuhi ʻana e like me ke kuhikuhi no ka hoʻohana ʻana. ʻO ka nui o ka unuhi ʻana i manaʻo ʻia o ka laʻana he 140µL, a ʻo ka nui o ka elution i manaʻo ʻia he 60µL.
