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    • Orientia tsutsugamushi

    ʻO Orientia tsutsugamushi Kiʻi ʻia
    • Orientia tsutsugamushi

    ʻO ka wehewehe pōkole:

    Hoʻohana ʻia kēia pahu no ka ʻike qualitative o ka nucleic acid o Orientia tsutsugamushi i nā laʻana serum.


    Huahana Huahana

    Huahana Huahana

    inoa huahana

    HWTS-OT002B Orientia tsutsugamushiʻO ka pahu ʻike ʻakika nucleic (Fluorescence PCR)

    Epidemiology

    ʻO ka scrub typhus kahi maʻi febrile koʻikoʻi i hoʻokumu ʻia e ka maʻi Orientia tsutsugamushi (Ot). ʻO ka Orientia scrub typhus kahi mea ʻokoʻa Gram-negative obligate intracellular parasitic microorganism. Aia ka Orientia scrub typhus i ka genus Orientia ma ke kauoha Rickettsiales, ka ʻohana Rickettsiaceae, a me ka genus Orientia. Hoʻouna ʻia ka scrub typhus ma o ka nahu ʻana o nā larvae chigger e lawe ana i nā pathogens. Ua hōʻike ʻia e ke kuni kiʻekiʻe koke, eschar, lymphadenopathy, hepatosplenomegaly, a me ka leukopenia koko peripheral, a me nā mea ʻē aʻe.

    Kanal

    FAM Orientia tsutsugamushi
    ROX

    Hoomalu Kuloko

    Nā ʻāpana ʻenehana

    Waihona

    ≤-18 ℃
    Kau-ola 12 mahina
    ʻAno Hoʻohālike serum hou
    Tt ≤38
    CV ≤5.0%
    LoD 500 kope/μL
    Nā mea hana pili Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli

    Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems

    QuantStudio®5 Pūnaehana PCR manawa maoli

    SLAN-96P Pūnaehana PCR manawa maoli (Hongshi Medical Technology Co., Ltd.)

    LightCycler®480 Pūnaehana PCR manawa maoli

    LineGene 9600 Plus Pūnaehana ʻIke PCR manawa maoli (FQD-96A, ʻenehana Hangzhou Bioer)

    MA-6000 Mea Hoʻokele Thermal Cycler Manawa maoli (Suzhou Molarray Co., Ltd.)

    ʻO BioRad CFX96 Pūnaehana PCR manawa maoli, BioRad CFX Opus 96 Pūnaehana PCR manawa maoli.

    Holo Hana

    Manaʻo ʻia ʻo reagent unuhi: Macro & Micro-TestGeneralaDNA/RNA Kit (HWTS-3019) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Pono e hoʻokō ʻia ka unuhi e like me ke kuhikuhi no ka hoʻohana ʻana i kēia reagent extraction. ʻO 200µL ka nui o ka laʻana i unuhi ʻia, a ʻo ka nui elution i ʻōlelo ʻia100µL.

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