ʻO Zaire Ebola Virus
inoa huahana
HWTS-FE008 Zaire Ebola Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
No Filoviridae ka ma'i Ebola, 'o ia ka ma'i ma'i ma'i ma'i ma'i 'ino-'ole i wae 'ia. ʻO nā maʻi virus he mau filament lōʻihi me ka awelika virion lōʻihi o 1000nm a me ke anawaena o kahi 100nm. ʻO ka genome maʻi Ebola he RNA ʻino-ʻole i hoʻokaʻawale ʻia me ka nui o 18.9kb, e hoʻopili ana i 7 mau protein kūkulu ʻia a me 1 protein non-structural. Hiki ke hoʻokaʻawale ʻia ka maʻi Ebola i nā ʻano like me Zaire, Sudan, Bundibugyo, Tai Forest a me Reston. Ma waena o lākou, ua hōʻike ʻia ke ʻano Zaire a me ke ʻano Sudan i mea e make ai ka poʻe he nui mai ka maʻi. ʻO EHF (Ebola Hemorrhagic Fever) he maʻi maʻi maʻi hemorrhagic i hoʻokumu ʻia e ka maʻi Ebola. Hoʻopili nui ʻia nā kānaka ma ka hoʻopili ʻana me nā wai kino, nā mea huna a me nā excreta o nā mea maʻi a i ʻole nā holoholona maʻi, a ʻo nā hōʻike lapaʻau ka nui o ke kuni, ke koko a me nā pōʻino kino he nui. He kiʻekiʻe ka nui o ka make o ka EHF ma 50%-90%.
Kanal
| FAM | ʻakika nucleic MP |
| ROX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 9 mahina |
| ʻAno Hoʻohālike | Serum hou, Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 kope/μL |
| kikoo | E hoʻohana i nā kits e hoʻāʻo i nā kuhikuhi maikaʻi ʻole o ka hui, kūpono nā hopena i nā koi. |
| Nā mea hana pili | Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR Manawa maoli SLAN-96P Pūnaehana PCR manawa maoli (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli (FQD-96A, ʻenehana Hangzhou Bioer) MA-6000 Mea Hoʻokele Thermal Cycler Manawa maoli (Suzhou Molarray Co., Ltd.) ʻO BioRad CFX96 Pūnaehana PCR manawa maoli, a me BioRad CFX Opus 96 Pūnaehana PCR manawa maoli. |
Holo Hana
Koho 1.
Manaʻo ʻia ka mea hoʻoulu hou: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction a i ʻole Purification Reagent (YDP315-R) na Tiangen Biotech (Beijing) Co., Ltd. Pono e unuhi ʻia e like me nā ʻōlelo aʻoaʻo, a ʻo ka nui o ka unuhi ʻana o ka hāpana ʻo 140μL a ʻo ka nui elution i ʻōlelo ʻia he 60μL.
Koho 2.
Manaʻo ʻia ka unuhi ʻana: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Pono e unuhi ʻia e like me nā kuhikuhi. ʻO 200μL ka nui o ka laʻana, a ʻo ka nui elution i ʻōlelo ʻia he 80μL.
Koho 3.
Manaʻo ʻia nā reagents unuhi: Nucleic Acid Extraction Reagent (1000020261) a me High Throughput Automated Sample Preparation System (MGISP-960) e BGI pono e unuhi ʻia e like me nā kuhikuhi. ʻO ka nui o ka unuhi ʻana he 160μL, a ʻo ka nui elution i ʻōlelo ʻia he 60μL.
