Ka maʻi Ebola o Zaire
Inoa huahana
Pahu ʻike waikawa nukleika o ka maʻi Ebola ʻo Zaire HWTS-FE008 (Fluorescence PCR)
ʻAlapia
No Filoviridae ka maʻi Ebola, he maʻi RNA kaula hoʻokahi ʻole i hoʻokaʻawale ʻia. He mau filaments lōʻihi nā maʻi me ka lōʻihi virion awelika o 1000nm a me ke anawaena o kahi o 100nm. ʻO ka genome maʻi Ebola he RNA kaula ʻole i hoʻokaʻawale ʻia me ka nui o 18.9kb, e hoʻopili ana i 7 mau protein kūkulu a me 1 protein ʻaʻole kūkulu. Hiki ke hoʻokaʻawale ʻia ka maʻi Ebola i nā ʻano e like me Zaire, Sudan, Bundibugyo, Tai Forest a me Reston. I waena o lākou, ua hōʻike ʻia ke ʻano Zaire a me ke ʻano Sudan e make ai nā kānaka he nui mai ka maʻi. ʻO ka EHF (Ebola Hemorrhagic Fever) kahi maʻi maʻi hemorrhagic acute i hoʻokumu ʻia e ka maʻi Ebola. Hoʻopili nui ʻia nā kānaka ma o ka hoʻopili ʻana me nā wai o ke kino, nā mea huna a me nā ʻōpala o nā maʻi a i ʻole nā holoholona i loaʻa i ka maʻi, a ʻo nā hōʻike lapaʻau e puka nui ana ke kuni, ke kahe ʻana o ke koko a me ka hōʻino ʻana o nā ʻōpū he nui. He kiʻekiʻe ka make ʻana o ka EHF he 50%-90%.
Kanal
| ʻOhana | Waikawa nucleic MP |
| ROX | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤-18℃ |
| Ola papa | 9 mahina |
| ʻAno Laʻana | Serum hou, Plasma |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Kope/μL |
| Kūlana kikoʻī | E hoʻohana i nā pahu hana e hoʻāʻo ai i nā kuhikuhi maikaʻi ʻole o ka ʻoihana, hoʻokō nā hopena i nā koi. |
| Nā Mea Hana Pili | Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli QuantStudio®5 Nā ʻōnaehana PCR Manawa Maoli SLAN-96P (Hongshi Medical Technology Co., Ltd.) ʻŌnaehana PCR Manawa Maoli LightCycler®480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus (FQD-96A, ʻenehana Hangzhou Bioer) MA-6000 Mea Hoʻopololei Wela Manawa Maoli (Suzhou Molarray Co., Ltd.) ʻŌnaehana PCR Manawa Maoli BioRad CFX96, a me ka ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
Koho 1.
ʻO ka mea hoʻohuihui i ʻōlelo ʻia: QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction or Purification Reagent (YDP315-R) na Tiangen Biotech (Beijing) Co.,Ltd. Pono e unuhi ʻia e like me nā kuhikuhi, a ʻo ka nui o ka unuhi ʻana o ka laʻana he 140μL a ʻo ka nui o ka elution i ʻōlelo ʻia he 60μL.
Koho 2.
ʻO ka mea hoʻohuihui i ʻōlelo ʻia: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Pono e unuhi ʻia e like me nā kuhikuhi. ʻO ka nui o ka laʻana unuhi he 200μL, a ʻo ka nui o ka elution i ʻōlelo ʻia he 80μL.
Koho 3.
Nā mea hoʻohuihui i ʻōlelo ʻia: Pono e hoʻohuihui ʻia ka Nucleic Acid Extraction Reagent (1000020261) a me ka High Throughput Automated Sample Preparation System (MGISP-960) e BGI e like me nā kuhikuhi. ʻO ka nui o ka hoʻohuihui he 160μL, a ʻo ka nui o ka elution i ʻōlelo ʻia he 60μL.
