Hoʻomaloʻo ʻia i ka hau 11 ʻAno o nā pathogens hanu Nucleic Acid
Inoa huahana
HWTS-RT190 -Paʻahau-maloʻo-Paʻahau-maloʻo 11 ʻAno o nā pathogens hanu Pahu ʻike waikawa Nucleic (Fluorescence PCR)
ʻAlapia
ʻO ka maʻi ʻōpū hanu kahi maʻi koʻikoʻi e hoʻoweliweli nui ana i ke olakino kanaka. Ua hōʻike ʻia nā haʻawina ua hoʻokumu ʻia ka hapa nui o nā maʻi ʻōpū hanu e nā pathogens bacteria a/a i ʻole viral e hoʻopili pū ana i ka mea hoʻokipa, e alakaʻi ana i ka hoʻonui ʻia o ka maʻi a i ʻole ka make. No laila, hiki i ka ʻike ʻana i ka pathogen ke hāʻawi i ka mālama kikoʻī a hoʻomaikaʻi i ka helu ola o ka mea maʻi [1,2]. Eia nō naʻe, ʻo nā ʻano kuʻuna no ka ʻike ʻana i nā pathogens hanu e komo pū me ka nānā ʻana microscopic, ka moʻomeheu bacteria, a me ka nānā ʻana immunological. He paʻakikī kēia mau ʻano hana, hoʻopau manawa, koi ʻenehana, a he haʻahaʻa ka naʻau. Eia kekahi, ʻaʻole hiki iā lākou ke ʻike i nā pathogens he nui i loko o kahi laʻana hoʻokahi, e paʻakikī ai ka hāʻawi ʻana i nā kauka i ka hōʻailona kōkua pololei. ʻO ka hopena, aia nō ka hapa nui o nā lāʻau lapaʻau i ke kahua lāʻau lapaʻau empirical, ʻaʻole wale ia e hoʻolalelale i ke kaʻina o ke kūʻē ʻana i ka bacteria, akā hoʻopilikia pū kekahi i ka hōʻailona manawa o nā maʻi [3]. ʻO ka Haemophilus influenzae maʻamau, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae, Stenotrophomonas maltophilia, Bordetella pertussis, Bordetella parapertussis, Mycoplasma pneumoniae, Chlamydia pneumoniae, a me Legionella pneumophila he mau pathogens koʻikoʻi e hoʻoulu ai i nā maʻi ʻōpū hanu nosocomial [4,5]. ʻIke kēia pahu hoʻāʻo a ʻike i nā waikawa nucleic kikoʻī o nā pathogens i luna i nā poʻe me nā hōʻailona a me nā ʻōuli o ka maʻi hanu, a hoʻohui pū me nā hopena keʻena hoʻokolohua ʻē aʻe e kōkua i ka ʻike ʻana i ka maʻi pathogen hanu.
Nā Palena ʻenehana
| Ka Waihona | 2-30℃ |
| Ola papa | 12 mahina |
| ʻAno Laʻana | ʻUhi ʻāʻī |
| Ct | ≤33 |
| CV | <5.0% |
| LoD | ʻO ka LoD o ke kime no Klebsiella pneumoniae he 500 CFU/mL; ʻo ka LoD o Streptococcus pneumoniae he 500 CFU/mL; ʻo ka LoD o Haemophilus influenzae he 1000 CFU/mL; ʻo ka LoD o Pseudomonas aeruginosa he 500 CFU/mL; ʻo ka LoD o Acinetobacter baumannii he 500 CFU/mL; ʻo ka LoD o Stenotrophomonas maltophilia he 1000 CFU/mL; ʻo ka LoD o Bordetella pertussis he 500 CFU/mL; ʻo ka LoD o Bordetella parapertussis he 500 CFU/mL; ʻo ka LoD o Mycoplasma pneumoniae he 200 kope/mL; ʻo ka LoD o Legionella pneumophila he 1000 CFU/mL; ʻO ka LoD o Chlamydia pneumoniae he 200 kope/mL. |
| Kūlana kikoʻī | ʻAʻohe hopena keʻa ma waena o ka pahu a me nā maʻi hanu maʻamau ʻē aʻe ma waho o ka pae ʻike o ka pahu hoʻāʻo, e like me Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Serratia marcescens, Enterococcus faecalis, Candida albicans, Klebsiella oxytoca, Streptococcus pyogenes, Micrococcus luteus, Rhodococcus equi, Listeria monocytogenes, Acinetobacter junii, Haemophilus parainfluenzae, Legionella dumov, Enterobacter aerogenes, Haemophilus haemolyticus, Streptococcus salivarius, Neisseria meningitidis, Mycobacterium tuberculosis, Influenza A virus, Influenza B virus, Aspergillus flavus, Aspergillus terreus, Aspergillus fumigatus, Candida glabrata, a me Candida tropicalis. |
| Nā Mea Hana Pili | ʻAno I: Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Manawa Maoli PCR ʻōnaehana, Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Manawa Maoli wikiwiki PCR ʻōnaehana, QuantStudio®5 mau ʻōnaehana PCR Manawa Maoli, SLAN-96P Nā ʻōnaehana PCR Manawa Maoli (Hongshi Medical Technology Co., Ltd.), LightCycler®480 Nā ʻōnaehana PCR Manawa Maoli, ʻōnaehana ʻike PCR Manawa Maoli LineGene 9600 Plus (FQD-96A, ʻenehana Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), ʻōnaehana PCR Manawa Maoli BioRad CFX96, ʻōnaehana PCR Manawa Maoli BioRad CFX Opus 96. ʻAno II: ʻEudemonTMʻO AIO800 (HWTS-EQ007) na Jiangsu Macro & Micro-Test Med-Tech Co. Ltd. |
Kahe Hana
ʻAno I: Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. ua ʻōlelo ʻia no ka unuhi ʻana i ka laʻana a pono e hana ʻia nā ʻanuʻu ma hope e like me ka IFU o ka Kit.
