KRAS 8 Mutations Detection Kit (Fluorescence PCR)-RUO
Inoa huahana
KRAS 8 Mutations Detection Kit (Fluorescence PCR)-RUO
ʻAlapia
Ua ʻike ʻia nā hoʻololi kiko i loko o ka gene KRAS i loko o kekahi mau ʻano puʻupuʻu kanaka, ma kahi o 17% ~ 25% ka nui o ka hoʻololi ʻana i loko o ka puʻupuʻu, 15% ~ 30% ka nui o ka hoʻololi ʻana i nā maʻi maʻi kanesa māmā, 20% ~ 50% ka nui o ka hoʻololi ʻana i nā maʻi maʻi kanesa colorectal. No ka mea, aia ka protein P21 i hoʻopili ʻia e ka gene K-ras ma lalo o ke ala hōʻailona EGFR, ma hope o ka hoʻololi ʻana o ka gene K-ras, hoʻāla mau ʻia ke ala hōʻailona ma lalo a ʻaʻole i hoʻopilikia ʻia e nā lāʻau lapaʻau i kuhikuhi ʻia ma luna ma EGFR, e hopena ana i ka hoʻonui mau ʻana o nā cell malignant. ʻO nā hoʻololi ʻana i loko o ka gene K-ras e hāʻawi pinepine i ke kūʻē ʻana i nā mea hoʻopaʻa EGFR tyrosine kinase i nā maʻi maʻi kanesa māmā a me ke kūʻē ʻana i nā lāʻau antibody anti-EGFR i nā maʻi maʻi kanesa colorectal [1, 2, 3]. I ka makahiki 2008, ua hoʻopuka ka National Comprehensive Cancer Network (NCCN) i kahi alakaʻi hana lapaʻau no ke kanesa colorectal, kahi i kuhikuhi ai ʻo nā wahi mutation e hoʻoulu ai i ka hoʻoulu ʻia ʻana o K-ras aia ma nā codons 12 a me 13 o exon 2, a ua ʻōlelo ʻia e hiki ke hoʻāʻo ʻia nā maʻi āpau me ka maʻi kanesa colorectal metastatic holomua no ka mutation K-ras ma mua o ka mālama ʻana [4]. No laila, ʻo ka ʻike wikiwiki a pololei o ka mutation gene K-ras he mea nui loa ia i ke alakaʻi lāʻau lapaʻau. Hoʻohana kēia pahu i ka DNA ma ke ʻano he laʻana ʻike e hāʻawi i ka loiloi qualitative o ke kūlana mutation, hiki ke kōkua i nā kauka i ka nānā ʻana i ke kanesa colorectal, ke kanesa māmā a me nā maʻi tumor ʻē aʻe e pōmaikaʻi ana mai nā lāʻau i kuhikuhi ʻia. ʻO nā hopena hoʻāʻo o ka pahu no ka ʻike lapaʻau wale nō a ʻaʻole pono e hoʻohana ʻia ma ke ʻano he kumu hoʻokahi no ka mālama pilikino ʻana i nā maʻi. Pono nā kauka e hana i nā hoʻoholo piha ma nā hopena hoʻāʻo ma muli o nā mea e like me ke kūlana o ka mea maʻi, nā hōʻailona lāʻau, ka pane lapaʻau a me nā hōʻailona hoʻāʻo hale hana ʻē aʻe.
Nā Palena ʻenehana
| Ka Waihona | ≤-18℃ |
| Ola papa | 12 mahina |
| ʻAno Laʻana | nā ʻāpana pathological i hoʻokomo ʻia e ka paraffin kanaka |
| CV | ≤5.0% |
| LoD | a) Hiki i ka K-ras Reaction Buffer A a me ka K-ras Reaction Buffer B ke ʻike paʻa i ka 1% mutation rate ma lalo o 3ng/μL wild-type background; b) He mutation o 1 × 103Hiki ke ʻike paʻa ʻia nā kope/mL ma kahi ʻano hihiu o 1 × 105Nā kope/mL ke 1% ka nui o ka mutation; c) Ke hoʻāʻo ʻia ka ʻōlelo kuhikuhi LoD o ka hui SW3, ʻaʻohe waiwai Ct a i ʻole ka waiwai Ct = 0 o Reaction Buffer A a me Reaction Buffer B |
| Nā Mea Hana Pili | Nā ʻōnaehana PCR Manawa Maoli i hoʻopili ʻia ʻo Biosystems 7500, Nā ʻōnaehana PCR Manawa Maoli i hoʻopili ʻia ʻo Biosystems 7300, Nā ʻōnaehana PCR Manawa Maoli ʻo QuantStudio®5, ʻōnaehana PCR Manawa Maoli ʻo LightCycler® 480, ʻōnaehana PCR Manawa Maoli ʻo BioRad CFX96. |
Kahe Hana
Pono ka reagent akā ʻaʻole i hāʻawi ʻia:ʻAʻohe H2O o DNase/RNase, ethanol anhydrous. I ka hoʻāʻo ʻana i kahi hāpana ʻiʻo i hoʻokomo ʻia i ka paraffin, ua ʻōlelo ʻia e hoʻohana i ka QIAGEN's QIAamp DNA FFPE Tissue Kit (56404) a me ka Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) i hana ʻia e Tiangen Biotech (Beijing) Co., Ltd.
Nā mea hoʻopau e pono ai akā ʻaʻole i hāʻawi ʻia:Nā wēlau ʻaʻohe DNase/RNase, nā mīkina lima hoʻolei ʻole, ka paipu Centrifuge ʻaʻohe DNase/RNase, nā ʻāpana 8-paipu, centrifuge.
