Pahu ʻIke Waikawa Nucleic no ka maʻi Dengue maloʻo-hau I/II/III/IV (Fluorescence PCR)-RUO
Inoa huahana
Pahu ʻIke Waikawa Nucleic no ka maʻi Dengue maloʻo-hau I/II/III/IV (Fluorescence PCR)-RUO
ʻAlapia
ʻO ke kuni Dengue (DF), i hoʻoulu ʻia e ka maʻi denguevirus (DENV), ʻo ia kekahi o nā maʻi laha loa o ka arbovirus. ʻO kāna mea hoʻolaha e komo pū me Aedes aegypti lāua ʻo Aedes albopictus. ʻO ka DF ka mea nui ma nā wahi wela a me nā wahi subtropical. No ka flavivirus ma lalo o flaviviridae ʻo DENV, a hiki ke hoʻokaʻawale ʻia i 4 serotypes e like me ka antigen o ka ʻili. ʻO nā hōʻike lapaʻau o ka maʻi DENV e komo pū ana me ka ʻeha poʻo, ke kuni, ka nāwaliwali, ka hoʻonui ʻia ʻana o ka lymph node, leukopenia a me nā mea ʻē aʻe, a me ke kahe koko, ka haʻalulu, ka ʻeha ate a i ʻole ka make i nā hihia koʻikoʻi. I nā makahiki i hala iho nei, ua hāʻawi ka loli o ke aniau, ke kūlanakauhale, ka hoʻomohala wikiwiki ʻana o ka mākaʻikaʻi a me nā mea ʻē aʻe i nā kūlana wikiwiki a maʻalahi hoʻi no ka hoʻouna ʻana a me ka hoʻolaha ʻana o DF, e alakaʻi ana i ka hoʻonui mau ʻana o ka wahi ahulau o DF. No laila, ʻo ka hoʻokumu ʻana i ke ʻano maʻalahi, kikoʻī a wikiwiki hoʻi o DENV he mea nui ia no ka hōʻoia lapaʻau o DF.
Nā Palena ʻenehana
| Ka Waihona | 2-8℃ |
| Ola papa | 12 mahina |
| ʻAno Laʻana | serum |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Kope/mL |
| Kūlana kikoʻī | He maikaʻi ʻole nā hoʻāʻo no nā laʻana serum maikaʻi ʻole o ka maʻi dengue; hōʻike nā hopena hoʻāʻo hoʻopilikia i ka wā ʻaʻole ʻoi aku ka nui o ka bilirubin i loko o ke koko ma mua o 168.2μmol/mL, ʻaʻole ʻoi aku ka nui o ka hemoglobin i hana ʻia e ka hemolysis ma mua o 130g/L, ʻaʻole ʻoi aku ka nui o ka lipid koko ma mua o 65mmol/mL, a ʻaʻole ʻoi aku ka nui o ka IgG i loko o ke koko ma mua o 5mg/mL, ʻaʻohe hopena i ka ʻike ʻana i ka maʻi dengue; e hoʻohana i ka maʻi Hepatitis A, ka maʻi Hepatitis B, ka maʻi Hepatitis C, ka maʻi Herpes, ka maʻi Eastern equine encephalitis, ka maʻi Hantaan, ka maʻi Bunia, ka maʻi West Nile, ka maʻi Zika a me nā laʻana serum genomic kanaka no ka hoʻāʻo cross-reactivity, a hōʻike nā hopena ʻaʻohe hopena cross ma waena o kēia kit a me nā pathogens ma luna. |
| Nā Mea Hana Pili | ʻAno I: Nā ʻōnaehana PCR Manawa Maoli Biosystems i hoʻopili ʻia 7500, nā ʻōnaehana PCR Manawa Maoli QuantStudio®5, nā ʻōnaehana PCR Manawa Maoli SLAN-96P (Hongshi Medical Technology Co., Ltd.), ʻōnaehana ʻike PCR Manawa Maoli LineGene 9600 Plus (FQD-96A, ʻenehana Hangzhou Bioer), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.), ʻōnaehana PCR Manawa Maoli BioRad CFX96, ʻōnaehana PCR Manawa Maoli BioRad CFX Opus 96. ʻAno II: ʻEudemonTMʻO AIO800 (HWTS-EQ007) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
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Kahe Hana
Pono nā reagents akā ʻaʻole i hāʻawi ʻia:
ʻAno I: Macro & Micro-Test General DNA/RNA Kit (HWTS-3017) (hiki ke hoʻohana ʻia me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd., Macro & Micro-Test Viral DNA/RNA Column (HWTS-3022).
ʻAno II: Macro & Micro-Test General DNA/RNA Kit (HWTS-3023-8), hiki ke hoʻohana ʻia me EudemonTM AIO800 (HWTS-EQ007) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ma mua o ka hoʻohana ʻana i ka reagent hoʻāʻo Type II, loaʻa i ka mea kūʻai aku kahi reagent unuhi kākoʻo a me ka mea hoʻonui, a e alakaʻi ka ʻenekinia i ka mea kūʻai aku e hoʻokō i ka hana hoʻokolohua i ka wā e hoʻohana ai i ka manawa mua.
Pono nā mea hoʻopau akā ʻaʻole i hāʻawi ʻia:Nā Manaʻo Kūpono ʻo DNase/RNase Free, nā mīkina lima hoʻolei ʻole, nā paipu DNase/RNase Free EP, centrifuge, kū magnetic, nā ʻāpana 8-paipu no PCR.
