Pahu ʻIke Hoʻololi Gene 29 EGFR Kanaka (Fluorescence PCR)-RUO
Inoa huahana
Pahu ʻIke Hoʻololi Gene 29 EGFR Kanaka (Fluorescence PCR)-RUO
ʻAlapia
Ua lilo ka maʻi kanesa māmā i kumu nui o ka make ʻana o ka maʻi kanesa ma ka honua holoʻokoʻa, e hoʻoweliweli nui ana i ke olakino kanaka. ʻO ka maʻi kanesa māmā ʻaʻole liʻiliʻi ke kelepona no kahi o 80% -85% o nā maʻi kanesa māmā. ʻO EGFR i kēia manawa ka pahuhopu molekala koʻikoʻi loa no ka mālama ʻana i ka maʻi kanesa māmā ʻaʻole liʻiliʻi. Hiki i ka phosphorylation o EGFR ke hoʻolaha i ka ulu ʻana o nā cell tumor, ka hoʻokaʻawale ʻana, ka hoʻouka kaua ʻana, ka metastasis, ka anti-apoptosis, a me ka hoʻolaha ʻana i ka angiogenesis tumor. Hiki i nā mea hoʻopaʻa tyrosine kinase EGFR (TKI) ke ālai i ke ala hōʻailona EGFR ma ka pale ʻana i ka autophosphorylation EGFR, a laila e pale ana i ka hoʻonui ʻana a me ka hoʻokaʻawale ʻana o nā cell tumor, e hoʻolaha ana i ka apoptosis cell tumor, e hōʻemi ana i ka angiogenesis tumor, a pēlā aku, i mea e hoʻokō ai i ka lāʻau lapaʻau i kuhikuhi ʻia i ka tumor [1]. Ua hōʻike ʻia he nui nā haʻawina e pili pono ana ka pono therapeutic o EGFR-TKI i ke kūlana o ka mutation gene EGFR, a hiki ke pale kūikawā i ka ulu ʻana o nā cell tumor me ka mutation gene EGFR [2,3]. Aia ka gene EGFR ma ka lima pōkole o ka chromosome 7 (7p12), me ka lōʻihi piha o 200Kb a he 28 exons. Aia ka ʻāpana mutation ma nā exons 18 a 21, ʻo nā codons 746 a 753 deletion mutation ma ka exon 19 e pili ana i kahi 45% a ʻo ka L858R mutation ma ka exon 21 e pili ana i kahi 40% a 45% [4]. Ua ʻōlelo maopopo nā Alakaʻi NCCN no ka ʻIke a me ka Lapaʻau ʻana o ka Non-Small Cell Lung Cancer e pono ka hoʻāʻo ʻana i ka mutation gene EGFR ma mua o ka hoʻokele ʻana o EGFR-TKI. Hoʻohana ʻia kēia pahu hoʻāʻo e alakaʻi i ka hoʻokele ʻana i nā lāʻau lapaʻau epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), a hāʻawi i ke kumu no ka lāʻau lapaʻau pilikino no nā poʻe maʻi me ka maʻi ʻaʻai māmā cell ʻole. Hoʻohana wale ʻia kēia pahu no ka ʻike ʻana i nā mutations maʻamau i loko o ka gene EGFR i nā poʻe maʻi me ka maʻi ʻaʻai māmā cell ʻole. No ka ʻōlelo lapaʻau wale nō nā hopena hoʻāʻo a ʻaʻole pono e hoʻohana ʻia ma ke ʻano he kumu hoʻokahi no ka mālama pilikino ʻana i nā poʻe maʻi. Pono nā kauka e noʻonoʻo i ke kūlana o ka mea maʻi, nā hōʻailona lāʻau, a me ka mālama ʻana. Hoʻohana ʻia ka hopena a me nā hōʻailona hoʻāʻo hale hana ʻē aʻe a me nā mea ʻē aʻe e hoʻoholo piha i nā hopena hoʻāʻo.
Kanal
| Hoʻopaʻa pane | Kanal |
| ʻO ka EGFR IC Reaction Buffer | Kanal FAM |
| L858R Reaction Buffer | Kanal FAM |
| 19del Reaction Buffer | Kanal FAM |
| Pākuʻi Hoʻihoʻi T790M | Kanal FAM |
| Hoʻopaʻa Manaʻo G719X | Kanal FAM |
| 3Ins20 Reaction Buffer | Kanal FAM |
| L861Q Reaction Buffer | Kanal FAM |
| Pākuʻi Hoʻihoʻi S768I | Kanal FAM |
Nā Palena ʻenehana
| Ka Waihona | Wai: ≤ -18 ℃ I ka pōʻeleʻele |
| Ola papa | 12 mahina |
| ʻAno Laʻana | ʻiʻo puʻupuʻu hou, ʻāpana pathological hau, ʻiʻo pathological i hoʻokomo ʻia i ka paraffin a i ʻole ka ʻāpana, plasma a serum paha |
| CV | <5.0% |
| LoD | Ma lalo o ke kua o ka 3ng/μL wild-type, ʻike ʻia ka hopena o ka hopena waikawa nucleic, hiki ke ʻike paʻa i ka 1% mutation rate; b) ka hopena waikawa nucleic Ma lalo o ka helu mutation 1%, hiki ke ʻike paʻa ʻia ka mutation 1 × 103Copys/mL ma ke kua ʻano hihiu o 1 × 105Copys/mL; c) Hiki i ka hopena hopena kuhikuhi kūloko ke ʻike i ka huahana kuhikuhi SW3 me ka palena ʻike haʻahaʻa loa o ka kuhikuhi kūloko o ka ʻoihana. Ke ʻike ka pahu i ka mea kuhikuhi palena ʻike aupuni, hiki ke ʻike paʻa ʻia ka mea kuhikuhi palena ʻike pinepine mutation 2.5% a me ka mutation hoʻonui gene EGFR me kahi ʻokoʻa helu kope o 4. |
| Kūlana kikoʻī | ʻAʻohe cross-reactivity me ka DNA genomic kanaka wild-type a me nā ʻano mutant ʻē aʻe |
| Nā Mea Hana Pili | Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Real-Time PCR Systems, Nā ʻōnaehana Biosystems i hoʻopili ʻia 7300 Nā ʻōnaehana PCR Manawa Maoli, ʻO QuantStudio® 5 mau ʻōnaehana PCR Manawa Maoli, ʻŌnaehana PCR Manawa Maoli LightCycler® 480, ʻŌnaehana PCR Manawa Maoli ʻo BioRad CFX96. |
Kahe Hana
Pono nā reagents akā ʻaʻole i hāʻawi ʻia:ʻO ka wai manuahi ʻo DNase/RNase, ethanol anhydrous. I ka hoʻāʻo ʻana i kahi hāpana ʻiʻo i hoʻokomo ʻia i ka paraffin, ua ʻōlelo ʻia e hoʻohana i ka QIAamp DNA FFPE Tissue Kit (56404) na QIAGEN a me ka Paraffin-embedded Tissue DNA Rapid Extraction Kit (DP330) na Tiangen Biotech (Beijing) Co., Ltd.. I ka hoʻāʻo ʻana i ka hāpana plasma/serum, ua ʻōlelo ʻia e hoʻohana i ka Macro & Micro-Test General DNA/RNA Kit (HWTS-3019-50, HWTS-3019-32, HWTS-3019-48, HWTS-3019-96) (hiki ke hoʻohana ʻia me ka Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. a i ʻole QIAamp Circulating Nucleic Acid Kit (55114) na QIAGEN.
Pono nā mea hoʻopau akā ʻaʻole i hāʻawi ʻiaNā Manaʻo Kūpono ʻo DNase/RNase, nā mīkina lima hoʻolei, ka paipu Centrifuge DNase/RNase Free, nā ʻāpana 8-paipu no PCR, centrifuge.
