ʻO ka Mycobacterium Tuberculosis Nucleic Acid a me Rifampicin (RIF), Resistance (INH)

Kiʻi i hōʻike ʻia ʻo Mycobacterium Tuberculosis Nucleic Acid a me Rifampicin (RIF), Resistance (INH)

Wehewehe Pōkole:

Hoʻohana ʻia kēia pahu no ka ʻike ʻana i ka DNA Mycobacterium tuberculosis in vitro i loko o ka sputum kanaka, ka moʻomeheu paʻa (LJ Medium) a me ka moʻomeheu wai (MGIT Medium), ka wai lavage bronchial, a me nā mutations ma ka ʻāpana codon amino acid 507-533 (81bp, rifampicin resistance determing region) o ka gene rpoB o Mycobacterium tuberculosis rifampicin resistance, a me nā mutations ma nā wahi mutation nui o Mycobacterium tuberculosis isoniazid resistance. Hāʻawi ia i ke kōkua i ka ʻike ʻana i ka maʻi Mycobacterium tuberculosis, a ʻike ia i nā genes pale nui o rifampicin a me isoniazid, e kōkua ana i ka hoʻomaopopo ʻana i ke kūʻē ʻana o ka lāʻau lapaʻau o Mycobacterium tuberculosis i loaʻa i ka maʻi.

E leka uila mai iā mākou

Nā kikoʻī huahana

Nā Lepili Huahana

Inoa huahana

HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid a me Rifampicin (RIF), (INH) ʻIke Pahu (Melting Curve)

ʻAlapia

ʻO Mycobacterium tuberculosis, ʻo Tubercle bacillus (TB) paha, ʻo ia ka bacteria pathogenic e hoʻoulu ai i ka maʻi tuberculosis, a i kēia manawa, ʻo nā lāʻau lapaʻau anti-tuberculosis laina mua i hoʻohana pinepine ʻia e like me isoniazid, rifampicin a me ethambutol, a pēlā aku.^[1]Eia nō naʻe, ma muli o ka hoʻohana pono ʻole ʻana i nā lāʻau anti-tuberculosis a me nā ʻano o ke ʻano o ka paia cell o ka mycobacterium tuberculosis ponoʻī, ua hoʻomohala ka mycobacterium tuberculosis i ke kūʻē ʻana i nā lāʻau anti-tuberculosis, a ʻo kahi ʻano weliweli loa ʻo ia ka multidrug-resistant tuberculosis (MDR-TB), kahi i kūʻē i nā lāʻau ʻelua maʻamau a maikaʻi loa, ʻo rifampicin a me isoniazid.^[2].

Aia ka pilikia o ke kū'ē ʻana i nā lāʻau lapaʻau tuberculosis ma nā ʻāina āpau i noiʻi ʻia e ka WHO. I mea e hāʻawi ai i nā hoʻolālā lapaʻau pololei no nā poʻe maʻi tuberculosis, pono e ʻike i ke kū'ē ʻana i nā lāʻau anti-tuberculosis, ʻoiai ke kū'ē ʻana i ka rifampicin, kahi i lilo i hana diagnostic i ʻōlelo ʻia e ka WHO i ka mālama ʻana i ka maʻi tuberculosis.^[3]ʻOiai ua aneane like ka ʻike ʻia ʻana o ke kūʻē ʻana o ka rifampicin me ka ʻike ʻia ʻana o MDR-TB, ʻo ka ʻike wale ʻana i ke kūʻē ʻana o ka rifampicin e hoʻowahāwahā i nā poʻe maʻi me ka INH mono-resistant (e pili ana i ke kūʻē ʻana i ka isoniazid akā naʻe i ka rifampicin) a me ka rifampicin mono-resistant (naʻau i ka isoniazid akā kūʻē i ka rifampicin), hiki ke alakaʻi i nā poʻe maʻi i hoʻokau ʻia i nā regimen lapaʻau mua kūpono ʻole. No laila, ʻo nā hoʻāʻo kūʻē i ka isoniazid a me ka rifampicin nā koi haʻahaʻa loa i nā papahana kaohi DR-TB āpau.^[⁴^].

Nā Palena ʻenehana

Ka Waihona	≤-18℃
Ola papa	12 mahina
ʻAno Laʻana	Laʻana Sputum, Moʻomeheu Paʻa (LJ Medium), Moʻomeheu Wai (MGIT Medium)
CV	<5.0%
LoD	ʻO ka LoD o ka pahu no ka ʻike ʻana i ka Mycobacterium tuberculosis he 10 bacteria/mL;ʻo ka LoD o ka pahu no ka ʻike ʻana i ka rifampicin wild type a me ka mutant type he 150 bacteria/mL; ʻO ka LoD o ka pahu no ka ʻike ʻana i ka isoniazid wild type a me ka mutant type he 200 bacteria/mL.
Kūlana kikoʻī	1) ʻAʻohe hopena keʻa i ka wā e hoʻohana ai i ka pahu hana e ʻike i ka DNA genomic kanaka (500ng), nā ʻano pathogens hanu ʻē aʻe he 28, a me 29 mau ʻano mycobacteria non-tuberculous (e like me ka mea i hōʻike ʻia ma ka Papa 3).2) ʻAʻohe hopena keʻa i ka wā e hoʻohana ai i ka pahu hana e ʻike i nā wahi mutation o nā genes ʻē aʻe i kūʻē i ka lāʻau lapaʻau o ka rifampicin a me ka isoniazid sensitive Mycobacterium tuberculosis (e like me ka mea i hōʻike ʻia ma ka Papa 4).3) ʻO nā mea hoʻopilikia maʻamau i loko o nā laʻana e hoʻāʻo ʻia, e like me ka rifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide (45mg/L), zanamivir (0.5mg/L), dexamethasone (20mg/L) nā lāʻau lapaʻau, ʻaʻohe hopena i nā hopena hoʻāʻo kit.
Nā Mea Hana Pili	Nā ʻōnaehana PCR Manawa Maoli SLAN-96P (Hongshi Medical Technology Co., Ltd.), ʻŌnaehana PCR Manawa Maoli BioRad CFX96