ʻO ka Mycobacterium Tuberculosis Nucleic Acid a me Rifampicin(RIF), Kūʻē (INH)
inoa huahana
HWTS-RT147 Mycobacterium Tuberculosis Nucleic Acid and Rifampicin(RIF), (INH) Detection Kit (Melting Curve)
Epidemiology
ʻO ka Mycobacterium tuberculosis, i kapa koke ʻia ʻo Tubercle bacillus (TB), ʻo ia ka maʻi bacteria e hoʻoulu ai i ka maʻi maʻi maʻi, a i kēia manawa, ʻo nā lāʻau anti-tuberculosis laina mua i hoʻohana pinepine ʻia me isoniazid, rifampicin a me ethambutol, etc.[1]. Eia nō naʻe, ma muli o ka hoʻohana hewa ʻole ʻana o nā lāʻau anti-tuberculosis a me nā ʻano o ka pā hale o ka mycobacterium tuberculosis ponoʻī, ua hoʻomohala ʻia ka mycobacterium tuberculosis i ka lāʻau kūʻē i nā lāʻau anti-tuberculosis, a ʻo kahi ʻano pōʻino loa he multidrug-resistant tuberculosis (MDR-TB), ka mea kūʻē i nā lāʻau lapaʻau maʻamau a maikaʻi loa, rifampicin a me ka rifampicin.[2].
Aia ka pilikia o ke kū'ē ʻana i ka lāʻau maʻi maʻi ma nā ʻāina āpau i noiʻi ʻia e ka WHO. No ka hoʻolako ʻana i nā hoʻolālā lapaʻau pololei loa no nā maʻi maʻi maʻi maʻi, pono e ʻike i ke kūʻē ʻana i nā lāʻau anti-tuberculosis, ʻoi aku ka pale ʻana o rifampicin, i lilo i mea diagnostic i ʻōlelo ʻia e ka WHO i ka mālama ʻana i ka maʻi maʻi.[3]. ʻOiai ʻo ka loaʻa ʻana o ke kū'ē rifampicin ua aneane like ia me ka loaʻa ʻana o ka MDR-TB, ʻo ka ʻike ʻana i ke kū'ē rifampicin e nānā ʻole i ka poʻe maʻi me ka INH kūʻokoʻa (e pili ana i ke kū'ē ʻana i ka isoniazid akā pili i ka rifampicin) a me ka rifampicin mono-resistant (ʻike i ka isoniazid akā kū'ē i ka rifampicin), hiki ke alakaʻi i nā maʻi i hoʻopaʻa ʻia i ka lāʻau mua kūpono ʻole. No laila, ʻo ka hoʻāʻo kūʻē ʻana i ka isoniazid a me ka rifampicin he mau koi liʻiliʻi e pono ai i nā papahana hoʻomalu DR-TB āpau[4].
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | Laʻana Sputum, Moʻomeheu Paʻa (LJ Medium), Moʻomeheu Liquid (MGIT Medium) |
| CV | <5.0% |
| LoD | ʻO ka LoD o ka pahu no ka ʻike ʻana i ka Mycobacterium tuberculosis he 10 bacteria/mL;ʻO ka LoD o ka kit no ka ʻike ʻana i ke ʻano ʻāhiu rifampicin a me ke ʻano mutant he 150 bacteria/mL; ʻO ka LoD o ka kit no ka ʻike ʻana i ke ʻano hihiu isoniazid a me ke ʻano mutant he 200 bacteria/mL. |
| kikoo | 1) ʻAʻohe hopena keʻa i ka wā e hoʻohana ai i ka pahu e ʻike ai i ka DNA genomic kanaka (500ng), nā ʻano ʻē aʻe 28 o nā maʻi hanu hanu, a me 29 mau ʻano mycobacteria non-tuberculous (e like me ka hōʻike ʻana ma ka Papa 3).2) ʻAʻohe hopena keʻa i ka wā e hoʻohana ai i ka pahu e ʻike i nā wahi hoʻololi o nā genes kūʻē i ka lāʻau o rifampicin a me isoniazid sensitive Mycobacterium tuberculosis (e like me ka hōʻike ʻana ma ka Papa 4).3) Nā mea hoʻopilikia maʻamau i loko o nā laʻana e hoʻāʻo ʻia, e like me rifampicin (9mg/L), isoniazid (12mg/L), ethambutol (8mg/L), amoxicillin (11mg/L), oxymetazoline (1mg/L), mupirocin (20mg/L), pyrazinamide (45mg/L), (0mg/L) pyrazinamide (45mg/L) nā lāʻau lapaʻau, ʻaʻohe hopena i nā hopena hōʻike kit. |
| Nā mea hana pili | SLAN-96P Pūnaehana PCR manawa maoli (Hongshi Medical Technology Co., Ltd.), BioRad CFX96 Pūnaehana PCR Manawa maoli |
Huina PCR
