ʻO ke gene A/B toxin Clostridium difficile（C.diff）
Inoa huahana
Pahu ʻike waikawa nuklea HWTS-OT031A no ka gene toxin Clostridium difficile A/B (C.diff) (Fluorescence PCR)
Palapala hōʻoia
CE
ʻAlapia
ʻO Clostridium difficile (CD), kahi Clostridium difficile anaerobic gram-positive sporogenic, ʻo ia kekahi o nā pathogens nui e hoʻoulu ai i nā maʻi ʻōpū nosocomial. Ma ke ʻano lapaʻau, ma kahi o 15% ~ 25% o ka maʻi diarrhea e pili ana i ka antimicrobial, 50% ~ 75% o ka colitis e pili ana i ka antimicrobial a me 95% ~ 100% o ka pseudomembranous enteritis i hoʻokumu ʻia e ka maʻi Clostridium difficile (CDI). He pathogen conditional ʻo Clostridium difficile, me nā ʻano toxigenic a me nā ʻano non-toxigenic.
Kanal
| ʻOhana | tcdAʻiʻo |
| ROX | tcdBʻiʻo |
| VIC/HEX | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤-18℃ |
| Ola papa | 12 mahina |
| ʻAno Laʻana | noho |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| Kūlana kikoʻī | e hoʻohana i kēia pahu hana e ʻike i nā maʻi ʻōpū ʻē aʻe e like me Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile non-pathogenic strains, Adenovirus, rotavirus, norovirus, influenza A virus, influenza B virus a me ka DNA genomic kanaka, ua maikaʻi ʻole nā hopena a pau. |
| Nā Mea Hana Pili | Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 QuantStudio®5 mau ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR Manawa Maoli SLAN-96P (Hongshi Medical Technology Co., Ltd.) LightCycler®ʻŌnaehana PCR Manawa Maoli 480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 PlusFQD-96A，Hangzhouʻenehana Bioer) MA-6000 Mea Hoʻopololei Wela Manawa Maoli (Suzhou Molarray Co., Ltd.) ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
Koho 1.
E hoʻohui i 180μL o ka lysozyme buffer i ka precipitate (e hoʻoheheʻe i ka lysozyme i 20mg/mL me ka lysozyme diluent), pipette e hui maikaʻi, a hana ma 37°C no nā minuke he 30 a ʻoi. E lawe i 1.5mL o ka RNase/DNase-free centrifuge tube, a hoʻohui180μL o ka mana maikaʻi a me ka mana maikaʻi ʻole ma ke kaʻina. Hoʻohui10μL o ka mana kūloko i ka hāpana e hoʻāʻo ʻia, ka mana maikaʻi, a me ka mana maikaʻi ʻole ma ke kaʻina, a hoʻohana i ka Nucleic Acid Extraction a i ʻole Purification Reagent (YDP302) na Tiangen Biotech (Beijing) Co., Ltd. no ka unuhi ʻana o ka DNA hāpana ma hope, a e ʻoluʻolu e hahai pono i nā kuhikuhi no ka hoʻohana ʻana no nā ʻanuʻu kikoʻī. E hoʻohana i ka DNase/RNase free H2O no ka elution, a ʻo ka nui elution i ʻōlelo ʻia he 100μL.
Koho 2.
E lawe i 1.5mL o ka paipu centrifuge RNase/DNase-free, a hoʻohui i 200μL o ka mana maikaʻi a me ka mana maikaʻi ʻole ma ke kaʻina. E hoʻohui10μL o ka mana kūloko i ka hāpana e hoʻāʻo ʻia, ka mana maikaʻi, a me ka mana maikaʻi ʻole ma ke kaʻina, a e hoʻohana i ka Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Pono e hoʻokō ʻia ka unuhi ʻana e like me ke kuhikuhi no ka hoʻohana ʻana, a ʻo ka nui o ka elution i ʻōlelo ʻia he 80μL.
