Clostridium difficile toxin A/B gene（C.diff）
Inoa mea kūʻai
HWTS-OT031A Nucleic Acid Detection Kit no Clostridium difficile toxin A/B gene (C.diff) (Fluorescence PCR)
Palapala
CE
Epidemiology
ʻO Clostridium difficile (CD), kahi gram-positive anaerobic sporogenic Clostridium difficile, ʻo ia kekahi o nā pathogens nui e hoʻoulu ai i nā maʻi ʻōpū nosocomial.ʻO ka maʻi maʻi, ma kahi o 15% ~ 25% o ka maʻi maʻi e pili ana i ka antimicrobial, 50% ~ 75% o ka colitis pili antimicrobial a me 95% ~ 100% o ka pseudomembranous enteritis ke kumu o Clostridium difficile infection (CDI).ʻO Clostridium difficile kahi pathogen conditional, e komo pū me nā ʻano toxigenic a me nā ʻano mea ʻole-toxigenic.
Kanal
| FAM | tcdAgene |
| ROX | tcdBgene |
| VIC/HEX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | stool |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 200CFU/mL |
| kikoo | E hoʻohana i kēia pahu e ʻike i nā pathogens ʻē aʻe e like me Escherichia coli, Staphylococcus aureus, Shigella, Salmonella, Vibrio parahaemolyticus, Group B Streptococcus, Clostridium difficile non-pathogenic strains, Adenovirus, rotavirus, norovirus, influenza A virus, influenza B virus a me ke kanaka genomic ʻO DNA, maikaʻi ʻole nā hopena. |
| Nā mea hana pili | Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli Hoʻohana ʻia ʻo Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®5 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR manawa maoli (Hongshi Medical Technology Co., Ltd.) LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli(FQD-96A，Hangzhouʻenehana bioer) MA-6000 Mea Hoʻokele Thermal Cycler manawa maoli (Suzhou Molarray Co., Ltd.) BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Koho 1.
E hoʻohui i ka 180μL o ka lysozyme buffer i ka precipitate (dilute i ka lysozyme i 20mg / mL me ka lysozyme diluent), pipette e hui maikaʻi, a hana ma 37 ° C no nā minuke 30. E lawe i 1.5mL o RNase / DNase-free centrifuge tube, a hoʻohui180μL o ka mana maikaʻi a me ka mana maikaʻi ʻole i ke kaʻina.Hoʻohui10μL o ka mana o loko i ka hāpana e ho'āʻoʻia, ka mana maikaʻi, a me ka mana maikaʻiʻole i ke kaʻina, a hoʻohana i ka Nucleic Acid Extraction or Purification Reagent (YDP302) e Tiangen Biotech (Beijing) Co., Ltd. no ka hōʻiliʻili DNA hope, a e ʻoluʻolu e hahai i nā ʻōlelo kuhikuhi no ka hoʻohana ʻana no nā pae kikoʻī.E hoʻohana iā DNase/RNase manuahi H2ʻO no ka elution, a ʻo ka nui o ka elution i ʻōlelo ʻia he 100μL.
Koho 2.
E lawe i 1.5mL o RNase/DNase-free centrifuge tube, a hoʻohui i 200μL o ka mana maikaʻi a me ka mana maikaʻi ʻole i ke kaʻina.Hoʻohui10μL o ka mana o loko i ka hāpana e ho'āʻoʻia, ka mana maikaʻi, a me ka mana maikaʻiʻole i ke kaʻina, a hoʻohana i ka Macro & Micro-Test Viral DNA / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004- 96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).Pono e hoʻokō ʻia ka unuhi e like me ke aʻo ʻana no ka hoʻohana ʻana, a ʻo ka nui o ka elution i ʻōlelo ʻia he 80μL.
