Clostridium diffice diffin toxin a / b gy (c.diff)
Hua waihona
Hwts-OT031A NCUCLE ACIDECE ENCED UNDER EXFIFICIFILIE TEXIN A / B T.DIFTCEnce PCR)
HAAKAANA
CE
Epideminey
ʻO ka clostridium diffice (CD), kahi huapalapala o Anaerobic Sprotridium Frostridic,ʻo ia kekahi o nā Pathoge nui e pili ana i nā maʻi maʻi nosocomial. Clinically, e pili ana i ka 15% ~ 25% o ka maʻi Antimicrobial-pili e pili ana iā Antimicheaial ʻO ka clostridium diffice ma kahi o ka pathogen matchgen
Kauwe
| Ka ha | tcdaKau |
| Rox | tcdbKau |
| VIC / Hex | Kaohi kūloko |
Nā poʻoaʻenehana
| Huanui | ≤-18 ℃ |
| 'Āmaʻomaʻo | 12 mau mahina |
| ʻAno kiko'ī | Stool |
| Tt | ≤38 |
| CV | ≤5.0% |
| Lolulu | 200cfu / ml |
| Kiko'ī | E hoʻohana i kēia kukuna eʻike i nā pathogens'ē aʻe e like me esherlia, stashticlus DNA, nā hopena maikaʻiʻole nā hopena. |
| Nā mea pili | Noi biosysteystems 7500 mau'ōnaehana PCR Noi biosystems 7500 wikiwiki mau'ōnaehana PCR Qutudidio®5 mau'ōnaehana PCR SLAN-96P mea maoli-manawa PCR Systems (Hongshi lāʻau lapaʻauʻenehana co., Ltd.) Waiwai ka Lio®480ʻO ka'ōnaehana PCR manawa Laina linegene 9600 me ka'ōnaehana o PCR i ka manawaFqd -_a,Hangzhou'Techlelo Mīlani) Ma-6000 maoli-manawa nui i ka manawa nui Biorad CFX96 Nā Kūlana Kūlana Lacr Biorad CFX OPus 96 Reass PCR System |
Nā kahe o ka hana
Ke koho 1.
E hoʻohui i ka 180μl o ka lysomezyme buffer i ka hopena (Dilite i ka lysozyme i ka makahiki he 18mg / my centifudge a hoʻohui180μL o ka mālama maikaʻi a me ka mālama maikaʻiʻole i ka pauku. Hoʻohui10μl o ka mana o loko i ka sample e ho'āʻoʻia, ka mana kūpono, a me ka mana maikaʻiʻole ma ka chatech (yd402 Eʻoluʻolu e hahai i nā'ōlelo kuhikuhi no ka hoʻohana no nā hana kiko'ī. Hoʻohana dnase / rnase free h2O No ka leʻaleʻa, a me ka leo i'ōleloʻiaʻia he 100μl.
Koho 2.
E lawe iā 1.5ml o Rnase / Dnase-Free Centrifuge Tube, a hoʻohui i ka 200μL o ka mana kūpono a me ka mana maikaʻiʻole. Hoʻohui10μl o ke kāohi kūloko i keʻano hoʻohālike e ho'āʻoʻia, ka mana kūpono, a me ka mana maikaʻiʻoleʻo Macro-3004-32-HWTS-3004-3004- 96) a me Macro & Micro-micro-micro-microt ictictic nucleic acid extictor (HWTS-3006). Pono e laweʻia ka mea hana i loko o kahi e like me ke aʻoʻana no ka hoʻohana, aʻo ka moni i'ōleloʻia e ka nui o ka heʻuli no ka pauna ma ka 80μl.
