Ua hui pū ʻia nā pathogens hanu
Inoa huahana
Pahu ʻIke Hoʻohui ʻia ʻo HWTS-RT158A no nā maʻi hanu (Fluorescence PCR)
Palapala hōʻoia
CE
ʻAlapia
ʻO ka maʻi Corona Virus 2019, i kapa ʻia ʻo'COVID-19', e pili ana i ka maʻi pneumonia i hoʻokumu ʻia e ka maʻi 2019-nCoV. ʻO 2019-nCoV kahi coronavirus no ka genus β. He maʻi lele hanu koʻikoʻi ʻo COVID-19, a he maʻalahi ka heluna kanaka. I kēia manawa, ʻo ke kumu o ka maʻi ʻo ia ka poʻe maʻi i loaʻa i ka 2019-nCoV, a ʻo ka poʻe maʻi asymptomatic paha ke kumu o ka maʻi. Ma muli o ka noiʻi epidemiological o kēia manawa, ʻo ka manawa incubation he 1-14 mau lā, ʻo ka hapa nui he 3-7 mau lā. ʻO ke kuni, ka ʻū maloʻo a me ka luhi nā hōʻike nui. Ua loaʻa i kekahi mau maʻi nā hōʻailona e like me ka ihu paʻa, ka ihu wai, ka ʻeha ʻāʻī, ka myalgia a me ka diarrhea, a pēlā aku.
ʻO ka maʻi influenza, i ʻike nui ʻia ʻo "flu", he maʻi lele hanu ʻoi loa ia i hoʻokumu ʻia e ka maʻi influenza. He mea lele nui ia. Hoʻolaha nui ʻia ma o ke kuni ʻana a me ka ʻūhā ʻana. Hoʻomaka pinepine ia i ka pūnāwai a me ka hoʻoilo. Ua māhele ʻia nā maʻi influenza i ʻekolu ʻano influenza A (IFV A), influenza B (IFV B), a me Influenza C (IFV C), no ka maʻi sticky a pau, ke kumu o ka maʻi kanaka no nā maʻi influenza A a me B, he maʻi RNA hoʻokahi kaula, ʻāpana. ʻO ka maʻi influenza A kahi maʻi hanu ʻoi loa, me H1N1, H3N2 a me nā ʻano ʻē aʻe, kahi i maʻalahi i ka mutation a me ka outbreak ma ka honua holoʻokoʻa. ʻO "Shift" e pili ana i ka mutation o ka maʻi influenza A, e hopena ana i ka puka ʻana mai o kahi "subtype" virus hou. Ua māhele ʻia nā maʻi influenza B i ʻelua mau moʻokūʻauhau, ʻo Yamagata lāua ʻo Victoria. Loaʻa i ka maʻi influenza B ka antigenic drift, a pale aku ia i ka nānā ʻana a me ka hoʻopau ʻana o ka ʻōnaehana pale kanaka ma o kona mutation. Eia naʻe, ʻoi aku ka lohi o ka wikiwiki o ka ulu ʻana o ka maʻi influenza B ma mua o ka maʻi influenza A kanaka. Hiki i ka maʻi Influenza B ke hoʻoulu i nā maʻi hanu kanaka a alakaʻi i nā maʻi ahulau.
ʻO ka maʻi syncytial hanu (RSV) he maʻi RNA, no ka ʻohana paramyxoviridae. Hoʻolaha ʻia ia e nā kulu ea a me ka pili kokoke a ʻo ia ke kumu nui o ka maʻi ʻaʻai hanu haʻahaʻa i nā pēpē. Hiki i nā pēpē i loaʻa i ka RSV ke loaʻa i ka bronchiolitis koʻikoʻi a me ka pneumonia, e pili ana i ka hānō i nā keiki. Loaʻa i nā pēpē nā hōʻailona koʻikoʻi, me ke kuni nui, rhinitis, pharyngitis a me laryngitis, a laila bronchiolitis a me ka pneumonia. Hiki i kekahi mau keiki maʻi ke paʻakikī me ka otitis media, pleurisy a me myocarditis, a me nā mea ʻē aʻe. ʻO ka maʻi ʻaʻai hanu kiʻekiʻe ka hōʻailona nui o ka maʻi i nā pākeke a me nā keiki makua.
Kanal
| ʻOhana | SARS-CoV-2 |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| Quasar 705 | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | -18℃ |
| Ola papa | 12 mahina |
| ʻAno Laʻana | ʻUwila waha |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300 Kope/mL ʻO ka maʻi Influenza A/Influenza B/Respiratory syncytial virus: 500 Kope/mL |
| Kūlana kikoʻī | a) Hōʻike nā hopena cross-reactivity ʻaʻohe cross reaction ma waena o ka kit a me ka coronavirus kanaka SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, human metapneumovirus, enterovirus A, B, C, D, epstein-barr virus, measles virus, human cytomegalo virus, rotavirus, norovirus, parotitis virus, varicella-zoster virus, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, smoke aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci a me ka cryptococcus hou a me ka human genomic nucleic acid. b) Ka hiki ke pale aku i ke komo ʻana: koho i ka mucin (60mg/mL), 10% (v/v) o ke koko a me ka phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (me nā mea mālama) (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL), ʻo ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) a me tobramycin (0.6mg/mL) no ka hoʻāʻo hoʻopilikia, a hōʻike nā hopena ʻaʻohe hopena hoʻopilikia o nā mea hoʻopilikia me nā ʻano i ʻōlelo ʻia ma luna nei i nā hopena hoʻāʻo o nā pathogens. |
| Nā Mea Hana Pili | ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR manawa maoli ʻo Rotor-Gene Q 5plex HRM Platform |
