Hoʻohui ʻia nā mea maʻi hanu

ʻO ka wehewehe pōkole:

Hoʻohana ʻia kēia pahu no ka ʻike qualitative o nā pathogens hanu i loko o ka waikawa nucleic i unuhi ʻia mai nā laʻana oropharyngeal swab kanaka.

Hoʻohana ʻia kēia ʻano hoʻohālike no ka ʻike qualitative o 2019-nCoV, influenza A virus, influenza B virus a me respiratory syncytial virus nucleic acids i loko o nā ʻano oropharyngeal swab samples.

E hoʻouna i ka leka uila iā mākou

Huahana Huahana

Inoa mea kūʻai

HWTS-RT158A Hui Pū ʻIa Hoʻopaʻa Hoʻohāhā (Fluorescence PCR)

Palapala

Epidemiology

ʻO ka maʻi ʻo Corona Virus 2019, i kapa ʻia ʻo'COVID-19', e pili ana i ka maʻi pneumonia i hoʻokumu ʻia e ka maʻi 2019-nCoV.ʻO 2019-nCoV kahi coronavirus pili i ka genus β.ʻO COVID-19 kahi maʻi maʻi ʻeha hanu, a maʻalahi ka heluna kanaka.I kēia manawa, ʻo ke kumu o ka maʻi ʻo ia ka poʻe maʻi i loaʻa i ka maʻi 2019-nCoV, a ʻo ka poʻe maʻi asymptomatic paha ke kumu o ka maʻi.Ma muli o ka noiʻi epidemiological o kēia manawa, ʻo ka manawa incubation he 1-14 mau lā, ʻo ka hapa nui he 3-7 mau lā.ʻO ke kuni, ka ʻū maloʻo a me ka luhi nā hōʻike nui.Ua loaʻa i kekahi mau maʻi nā hōʻailona e like me ka nasal congestion, runny ihu, ʻeha ʻāʻī, myalgia a me ka diarrhea, etc.

ʻO ka maʻi influenza, i ʻike nui ʻia ʻo "flu", he maʻi ʻeha ʻeha i hoʻokumu ʻia e ka maʻi maʻi influenza.He ma'i lele loa.Hoʻouna nui ʻia ia e ka ʻu ʻana a me ke kihe ʻana.He mea maʻamau i ka pūnāwai a me ka hoʻoilo.Hoʻokaʻawale ʻia nā maʻi maʻi maʻi influenza A (IFV A), influenza B (IFV B), a me ka Influenza C (IFV C) ʻekolu ʻano, no ka maʻi maʻi sticky, kumu nui o ke kanaka no nā maʻi influenza A a me B, hoʻokahi ia. - stranded, ʻāpana RNA virus.ʻO ka maʻi Influenza A he maʻi hanu nui, e like me H1N1, H3N2 a me nā ʻano subtype ʻē aʻe, hiki ke hoʻololi a maʻi i ka honua holoʻokoʻa.ʻO ka "Shift" e pili ana i ka hoʻololi ʻana o ka maʻi maʻi influenza A, ka hopena i ka puka ʻana mai o kahi maʻi hou "subtype".Ua māhele ʻia nā maʻi maʻi influenza B i ʻelua laina, ʻo Yamagata a me Victoria.ʻO ka maʻi Influenza B wale nō ka antigenic drift, a pale aku i ka nānā ʻana a me ka hoʻopau ʻana o ka ʻōnaehana pale kanaka ma o kona hoʻololi ʻana.Eia naʻe, ʻoi aku ka lohi o ka hoʻololi ʻana o ka maʻi maʻi influenza B ma mua o ka maʻi influenza A kanaka.Hiki i ka ma'i Influenza B ke ho'oulu i ka ma'i hanu kanaka a alaka'i i ka ma'i ahulau.

ʻO ka maʻi maʻi hanu hanu (RSV) he maʻi RNA, no ka ʻohana paramyxoviridae.Hoʻouna ʻia ia e nā kulu lewa a me ka pilina pili a ʻo ia ka pathogen nui o ka maʻi ʻeha haʻahaʻa o ka ʻōpū o nā pēpē.Hiki i nā kamaliʻi i loaʻa i ka RSV ke hoʻomohala i ka bronchiolitis koʻikoʻi a me ka maʻi pneumonia, pili i ka hānō i nā keiki.Loaʻa i nā pēpē nā hōʻailona koʻikoʻi, e like me ke kuni kiʻekiʻe, rhinitis, pharyngitis a me ka laryngitis, a laila bronchiolitis a me ka maʻi pneumonia.Hiki ke paʻakikī kekahi mau keiki maʻi me ka otitis media, pleurisy a me ka myocarditis, a me nā mea ʻē aʻe.

Kanal

FAM	SARS-CoV-2
VIC(HEX)	RSV
CY5	IFV A
ROX	IFV B
Quasar 705	Hoomalu Kuloko

Nā ʻāpana ʻenehana

Waihona	-18 ℃
Kau-ola	12 mahina
ʻAno Hoʻohālike	ʻOropharyngeal swab
Ct	≤38
LoD	2019-nCoV: 300 kope/mL ʻO ka maʻi maʻi Influenza A/Maʻi maʻi influenza B/Maʻi hoʻomaʻamaʻa hanu: 500 Kopi/mL
kikoo	a) Hōʻike nā hualoaʻa cross-reactivity ʻaʻohe hopena kea ma waena o ka kit a me ka coronavirus kanaka SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus type 1, 2, 3, rhinovirus A, B, C, chlamydia pneumoniae, metapneumovirus kanaka, enterovirus A, B, C, D, ma'i ma'i epstein-barr, ma'i ma'i 'a'ai, ma'i ma'i cytomegalo kanaka, rotavirus, norovirus, ma'i parotitis, ma'i virus varicella-zoster, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus pyogenes, klebsiella pneumoniae, mycobacterium tuberculosis, uahi aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci a me ke kanaka genomiccleic nut. b) Hiki ke hoʻopilikia: koho i ka mucin (60mg/mL), 10% (v/v) o ke koko a me ka phenylephrine (2mg/mL), oxymetazoline (2mg/mL), sodium chloride (me nā preservatives) (20mg/mL) , beclomethasone (20mg/mL), dexamethasone (20mg/mL), flunisolide (20μg/mL), triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL) , histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), oseltamivir (60ng/mL), peramivir (1mg/mL), lopinavir (500mg/mL). ), ritonavir (60mg/mL), mupirocin (20mg/mL), azithromycin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) a me tobramycin (0.6mg/mL) ) no ka ho'āʻo 'ākeʻakeʻa, a hōʻike nā hopena ʻaʻohe mea hoʻopilikia i nā hopena hoʻāʻo o nā pathogens.
Nā mea hana pili	BioRad CFX96 Pūnaehana PCR Manawa maoli Rotor-Gene Q 5plex HRM Platform Pūnaehana PCR manawa maoli