Staphylococcus Aureus a me Methicillin-Resistant Staphylococcus Aureus (MRSA/SA)
Inoa mea kūʻai
HWTS-OT062 Staphylococcus Aureus a me Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) Nucleic Acid Detection Kit(Fluorescence PCR)
Palapala
CE
Epidemiology
ʻO Staphylococcus aureus kekahi o nā maʻi pathogenic koʻikoʻi o ka maʻi nosocomial.No ka staphylococcus 'o Staphylococcus aureus (SA) a he 'elele ia no ka ma'i bacteria Gram-positive, hiki ke ho'opuka i nā 'ano mea 'awa'awa a me nā enzyme invasive.Loaʻa i ka bacteria nā hiʻohiʻona o ka hoʻolaha ākea, ka pathogenicity ikaika a me ka nui o ke kū'ē kiʻekiʻe.ʻO ke ʻano nuclease Thermotable (nuc) kahi gene i mālama nui ʻia o staphylococcus aureus.
Kanal
| FAM | gene mecA kū'ē i ka methicillin |
| ROX | Hoomalu Kuloko |
| CY5 | staphylococcus aureus nuc gene |
Nā ʻāpana ʻenehana
| Waihona | ≤-18 ℃ & pale ʻia mai ka mālamalama |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | sputum, ʻili a me ka ʻiʻo palupalu nā mea maʻi, a me nā laʻana nasal swab |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL bacteria kū'ē i ka methicillin.Ke ʻike ka pahu i ka ʻōlelo LoD aupuni, hiki ke ʻike ʻia ka 1000/mL staphylococcus aureus. |
| kikoo | Hōʻike ka hoʻāʻo cross-reactivity ʻaʻohe o kēia kit me nā mea maʻi hanu ʻē aʻe e like me ka methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiumaniella protein. mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Nā mea hana pili | Hoʻohana ʻia Biosystems 7500 Pūnaehana PCR manawa maoli QuantStudio®5 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 ʻOiaʻiʻo ka nui o ka wela wela BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Koho 1.
Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. hiki ke hoʻohana me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS- 3006B).E hoʻohui i 200µL o ka paʻakai maʻamau i ka precipitate i hana ʻia, a pono e unuhi ʻia nā ʻanuʻu ma hope e like me nā kuhikuhi, a ʻo ka nui o ka elution i ʻōlelo ʻia he 80µL.
Koho 2.
ʻO Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. E hoʻohui i 1mL o ka paʻakai maʻamau i ka precipitate ma hope o ka holoi ʻana me ka saline maʻamau, a laila hui maikaʻi.ʻO Centrifuge ma 13,000r/min no 5 mau minuke, e wehe i ka supernatant (mālama ʻia 10-20µL o supernatant), a hahai i nā kuhikuhi no ka unuhi ʻana ma hope.
Manaʻo ʻia ka mea hoʻoheheʻe ʻoki: Nucleic Acid Extraction a i ʻole Purification Reagent (YDP302) na Tiangen Biotech (Beijing) Co., Ltd.Pono e hoʻokō pono ʻia ka unuhi ʻana e like me ka ʻanuʻu 2 o ka papa kuhikuhi.Manaʻo ʻia e hoʻohana i ka wai RNase a me DNase-free no ka elution me ka nui o 100µL.
