ʻO Staphylococcus Aureus a me ka Staphylococcus Aureus Kūʻē i ka Methicillin (MRSA/SA)
Inoa huahana
HWTS-OT062 Staphylococcus Aureus a me Methicillin-Resistant Staphylococcus Aureus (MRSA/SA) Pahu ʻIke Waikawa Nucleic (Fluorescence PCR)
Palapala hōʻoia
CE
ʻAlapia
ʻO Staphylococcus aureus kekahi o nā bacteria pathogenic koʻikoʻi o ka maʻi nosocomial. No ka staphylococcus aureus (SA) a he ʻelele ia o nā bacteria Gram-positive, hiki ke hana i nā ʻano mea ʻawahia a me nā enzymes invasive. Loaʻa i nā bacteria nā ʻano o ka hoʻolaha ākea, ka pathogenicity ikaika a me ka helu kū'ē kiʻekiʻe. ʻO ka gene nuclease thermostable (nuc) kahi gene i mālama nui ʻia o ka staphylococcus aureus.
Kanal
| ʻOhana | ka gene mecA kū'ē i ka methicillin |
| ROX | Mana Kūloko |
| CY5 | ʻO ka gene nuc staphylococcus aureus |
Nā Palena ʻenehana
| Ka Waihona | ≤-18 ℃ a pale ʻia mai ka mālamalama |
| Ola papa | 12 mahina |
| ʻAno Laʻana | nā laʻana o ka sputum, ka ʻili a me nā ʻiʻo palupalu, a me nā laʻana swab ihu |
| Ct | ≤36 |
| CV | ≤5.0% |
| LoD | 1000 CFU/mL staphylococcus aureus, 1000 CFU/mL bacteria kū'ē i ka methicillin. Ke ʻike ka pahu i ka kuhikuhi LoD aupuni, hiki ke ʻike ʻia ka 1000/mL staphylococcus aureus |
| Kūlana kikoʻī | Hōʻike ka hoʻāʻo cross-reactivity ʻaʻohe cross reactivity o kēia pahu me nā pathogens hanu ʻē aʻe e like me ka methicillin-sensitive staphylococcus aureus, coagulase-negative staphylococcus, methicillin-resistant staphylococcus epidermidis, pseudomonas aeruginosa, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, proteus mirabilis, enterobacter cloacae, streptococcus pneumoniae, enterococcus faecium, candida albicans, legionella pneumophila, candida parapsilosis, moraxella catarrhalis, neisseria meningitidis, haemophilus influenzae. |
| Nā Mea Hana Pili | Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli QuantStudio®5 mau ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR Manawa Maoli SLAN-96P LightCycler®ʻŌnaehana PCR Manawa Maoli 480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus MA-6000 Mea Hoʻopololei Wela Manawa Maoli ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
Koho 1.
Hiki ke hoʻohana ʻia ka Macro & Micro-Test Genomic DNA/RNA Kit (HWTS-3019) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. me ka Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B). E hoʻohui i 200µL o ka saline maʻamau i ka precipitate i hana ʻia, a pono e unuhi ʻia nā ʻanuʻu ma hope e like me nā kuhikuhi, a ʻo ka nui o ka elution i ʻōlelo ʻia he 80µL.
Koho 2.
ʻO ka Macro & Micro-Test Sample Release Reagent (HWTS-3005-8) na Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. E hoʻohui i 1mL o ka saline maʻamau i ka precipitate ma hope o ka holoi ʻana me ka saline maʻamau, a laila e kāwili maikaʻi. E centrifuge ma 13,000r/min no 5 mau minuke, e wehe i ka supernatant (e mālama i 10-20µL o ka supernatant), a e hahai i nā kuhikuhi no ka unuhi ʻana ma hope.
ʻO ka mea hoʻohuihui i ʻōlelo ʻia: Nucleic Acid Extraction or Purification Reagent (YDP302) na Tiangen Biotech (Beijing) Co., Ltd. Pono e hoʻokō pono ʻia ka unuhi ʻana e like me ka pae 2 o ka manual kuhikuhi. Manaʻo ʻia e hoʻohana i ka wai ʻole RNase a me DNase no ka elution me ka nui o 100µL.
