ʻO Staphylococcus Aureus a me Mibicillin
Hua waihona
Hwts-Ot062 Staphylococcus Aureus a me Mixcillin-resistant Staphylococcus AURDUS (MRERA / FLeroscence PCR)
HAAKAANA
CE
Epideminey
ʻO Staphylococcus Aureus kekahi o nā balala o Pathogenic nui o Pathogenic i ka maʻi nosocomial. Loaʻa iā Staphylococcus Aureus Loaʻa i ka Bucteria keʻano o ka hoʻolahaʻana i ka nui ākea, ikaika pathonicity a me ke kiʻekiʻe o ke kū'ē kiʻekiʻe. ʻO ka nui o ka thermostase gecle (nuc) he greative nui loa ia o Staphylococcus Aureus.
Kauwe
| Ka ha | ʻO ka Miniclillin-resistant Meca Gury |
| Rox | Kaohi kūloko |
| Kūpono Kakau | Staphylococcus Aureus nuc gen |
Nā poʻoaʻenehana
| Huanui | ≤-18 ℃ & paleʻia mai ka mālamalama |
| 'Āmaʻomaʻo | 12 mau mahina |
| ʻAno kiko'ī | ʻO Sputum,ʻili a me nāʻili piha piha a me ka palupalu |
| Ct | ≤36 |
| CV | ≤5.0% |
| Lolulu | 1000 CFU / ML Staphylococcus Aureus, 1000 CFU / ML MiTichillin-resistant Baicteria. I kaʻikeʻana o ka pahu i keʻano o keʻano o ke aupuni, he 1000 / ml staphylococcus areusus hiki keʻikeʻia |
| Kiko'ī | ʻO ka hōʻike hōʻike hōʻike RED-REACTY i hōʻikeʻia i kēia kī i ka wikiwikiʻana i nā pahū e like me nā saldillin,ʻo Scapsilllas Aerice Acifotoceracterage Bamania, Protelubois, Poramorleloʻo FigIctior Pnuehuke, Meranikola Phoenikos, NectionalOla Pneumsilos, Meranicolusis |
| Nā mea pili | Noi biosysteystems 7500 mau'ōnaehana PCR Qutudidio®5 mau'ōnaehana PCR SLAN-96P mea maoli maoli PCR Waiwai ka Lio®480ʻO ka'ōnaehana PCR manawa Linegene 9600 a me ka'ōnaehanaʻo PCR maoli Ma-6000 maoli-manawa nui ka manawa nui Biorad CFX96 Nā Kūlana Kūlana Lacr Biorad CFX OPus 96 Reass PCR System |
Nā kahe o ka hana
Ke koho 1.
Macro & Micro-set GenMic DNA / RNA Kit (HWTS-3019) E hoʻohanaʻia me ka Macro-Text-3006C, HWTS- 3006b). E hoʻohui i ka 200μl o ka paʻakai maʻamau i ke kaʻina hana i hanaʻia, aʻo nā pae aʻe e hiki ke kiʻiʻia e like me nā'ōlelo e like me ka nui o nā'ōiwi,
Koho 2.
ʻO Macro & Micro-Micro-Micro-Micro-3005-8) e ka coarine ma hope o ka holoiʻana me ka holoi maʻamau ʻO ka Centifuge ma 13,000r / min no 5 mau minuke, e wehe i ka supernatant (Reserve 10-20μl no Supernatant.
Hoʻonohonohoʻia ka uku houʻana: Nuclec AcID Extraction Account a iʻole ka uku uku (YDP32) e Tiangen Biotech (Beijing) Co., LTD. Pono e laweʻia ka extration e like me keʻano o ka hana 2 o ke kauoha aʻo. Ua manaʻoʻia e hoʻohana i ka Rnase a me ka wai-dnase-free-free no ka leʻaleʻa me ka nui o ka nui o 100μl.
