ʻO ka Enterococcus kū'ē i ka Vancomycin a me ka Gene kū'ē i ka lāʻau lapaʻau
Inoa huahana
HWTS-OT090-Pahu ʻIke Gene Enterococcus kūʻē i ka Vancomycin a me nā lāʻau lapaʻau (Fluorescence PCR)
ʻAlapia
ʻIke ʻia hoʻi ke kūʻē ʻana i ka lāʻau lapaʻau ʻo ke kūʻē ʻana i ka lāʻau lapaʻau, e pili ana i ke kūʻē ʻana o ka bacteria i ka hana a nā lāʻau antibacterial. Ke kū mai ke kūʻē ʻana i ka lāʻau lapaʻau, e emi nui ka hopena chemotherapy o nā lāʻau lapaʻau. Ua māhele ʻia ke kūʻē ʻana i ka lāʻau lapaʻau i ke kūʻē kūloko a me ke kūʻē i loaʻa. Hoʻoholo ʻia ke kūʻē kūloko e nā genes chromosomal bacteria, i hoʻoili ʻia mai kēlā hanauna a i kēia hanauna, a ʻaʻole e loli. ʻO ke kūʻē i loaʻa ma muli o ka ʻoiaʻiʻo ma hope o ka launa ʻana me nā antibiotics, hoʻololi ka bacteria i ko lākou mau ala metabolic ponoʻī i ʻole lākou e make e nā antibiotics.
ʻO nā genes kū'ē vancomycin ʻo VanA a me VanB he kū'ē lāʻau i loaʻa, ma waena o ia mau mea e hōʻike ana ʻo VanA i nā pae kiʻekiʻe o ke kū'ē i ka vancomycin a me ka teicoplanin, hōʻike ʻo VanB i nā pae like ʻole o ke kū'ē i ka vancomycin, a he naʻau koʻikoʻi i ka teicoplanin. Hoʻohana pinepine ʻia ʻo Vancomycin i ka lāʻau lapaʻau e mālama i nā maʻi bacteria Gram-positive, akā ma muli o ka puka ʻana mai o nā enterococci kū'ē vancomycin (VRE), ʻoiai ʻo enterococcus faecalis a me enterococcus faecium, e helu ana ma mua o 90%, ua lawe mai ia i nā pilikia nui hou i ka lāʻau lapaʻau. I kēia manawa, ʻaʻohe lāʻau antibacterial kikoʻī no ka mālama ʻana i ka VRE. Eia kekahi, hiki iā VRE ke hoʻoili i nā genes kū'ē lāʻau i nā enterococci ʻē aʻe a i ʻole nā bacteria Gram-positive ʻē aʻe.
Kanal
| ʻOhana | ʻO ka enterococci kū'ē i ka Vancomycin (VRE): ʻO Enterococcus faecalis a me Enterococcus faecium |
| VIC/HEX | Mana Kūloko |
| CY5 | ʻO ke gene kū'ē vancomycin ʻo VanB |
| ROX | ʻO ke kine kū'ē vancomycin ʻo VanA |
Nā Palena ʻenehana
| Ka Waihona | Wai: ≤-18 ℃ |
| Ola papa | 12 mahina |
| ʻAno Laʻana | sputum, koko, mimi a i ʻole nā kolone maʻemaʻe |
| CV | ≤5.0% |
| Ct | ≤36 |
| LoD | 103CFU/mL |
| Kūlana kikoʻī | ʻAʻohe cross-reactivity me nā pathogens hanu ʻē aʻe e like me klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, streptococcus pneumoniae, neisseria meningitidis, staphylococcus aureus, klebsiella oxytoca, haemophilus influenzae, A. junii, A. haemolyticus, legionella pneumophila, escherichia coli, pseudomonas fluorescens, candida albicans, chlamydia pneumoniae, respiratory adenovirus, a i ʻole nā laʻana i loaʻa nā genes pale lāʻau ʻē aʻe CTX, mecA, SME, Nā laʻana o SHV a me TEM. |
| Nā Mea Hana Pili | ʻŌnaehana PCR Manawa Maoli Biosystems 7500 i hoʻopili ʻia Nā ʻōnaehana PCR Manawa Maoli SLAN-96P ʻŌnaehana PCR Manawa Maoli LightCycler®480 |
Kahe Hana
Nā mea hoʻohuihui i ʻōlelo ʻia: Macro & Micro-Test Genomic DNA Kit (HWTS-3014-32, HWTS-3014-48, HWTS-3014-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B).
