Ka Hoʻololi ʻana o ke Kine Hoʻohui TEL-AML1 Kanaka
Inoa huahana
Pahu ʻIke Hoʻololi Gene Fusion TEL-AML1 Kanaka HWTS-TM016 (Fluorescence PCR)
ʻAlapia
ʻO ka leukemia lymphoblastic acute (ALL) ka maʻi ʻino maʻamau i ka wā kamaliʻi. I nā makahiki i hala iho nei, ua loli ka leukemia acute (AL) mai ke ʻano MIC (morphology, immunology, cytogenetics) i ke ʻano MICM (hoʻohui ʻia o ka hoʻāʻo ʻana i ka biology molecular). I ka makahiki 1994, ua ʻike ʻia ua hoʻokumu ʻia ka TEL fusion i ka wā kamaliʻi e ka nonrandom chromosomal translocation t(12;21)(p13;q22) ma ka B-lineage acute lymphoblastic leukemia (ALL). Mai ka ʻike ʻia ʻana o ka gene fusion AML1, ʻo ka gene fusion TEL-AML1 ke ala maikaʻi loa e hoʻoholo ai i ka prognosis o nā keiki me ka leukemia lymphoblastic acute.
Kanal
| ʻOhana | ʻO ka gene fusion TEL-AML1 |
| ROX | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤-18℃ |
| Ola papa | 9 mahina |
| ʻAno Laʻana | hāpana iwi |
| Ct | ≤40 |
| CV | <5.0% |
| LoD | 1000 Kope/mL |
| Kūlana kikoʻī | ʻAʻohe cross-reactivity ma waena o nā kits a me nā genes fusion ʻē aʻe e like me BCR-ABL, E2A-PBX1, MLL-AF4, AML1-ETO, PML-RARa fusion genes. |
| Nā Mea Hana Pili | Nā ʻōnaehana Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR manawa maoli wikiwiki Biosystems i hoʻopili ʻia 7500 Nā ʻōnaehana PCR Manawa Maoli QuantStudio®5 Nā ʻōnaehana PCR Manawa Maoli SLAN-96P ʻŌnaehana PCR Manawa Maoli LightCycler®480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus MA-6000 Mea Hoʻopololei Wela Manawa Maoli ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
RNAprep Pure Blood Total RNA Extraction Kit (DP433).
