Zika Virus
inoa huahana
HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Palapala
CE
Epidemiology
No ka genus Flaviviridae ka ma'i Zika, he ma'i ma'i RNA i ho'opa'a 'ia me ka anawaena o 40-70nm. He envelop kona, aia 10794 nucleotides, a hoʻopili i 3419 amino acids. Wahi a ka genotype, ua māhele ʻia i ke ʻano ʻApelika a me ke ʻano ʻAsia. ʻO ka maʻi maʻi Zika kahi maʻi maʻi maʻi e hoʻemi ʻia e ka maʻi maʻi Zika, ka mea i lawe nui ʻia ma o ka nahu ʻana o nā makika Aedes aegypti. ʻO nā hiʻohiʻona o ka maʻi he maʻi kuni, ʻūlū, arthralgia a i ʻole conjunctivitis, a ʻaʻohe mea make. Wahi a ka World Health Organization, hiki ke pili i ka microcephaly neonatal a me Guillain-Barre syndrome (Guillain-Barré syndrome) me ka maʻi maʻi Zika.
Kanal
| FAM | Zika virus nucleic acid |
| ROX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | ≤30 ℃ & pale ʻia mai ka mālamalama |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | serum hou |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 kope/mL |
| kikoo | E hoʻohana i ka kit e ʻike i nā laʻana serum me ka maʻi maʻi Zika ʻino, a maikaʻi ʻole nā hopena. Hōʻike nā hopena hōʻike hoʻopilikia i ka wā ʻaʻole ʻoi aku ka nui o ka bilirubin i ka serum ma mua o 168.2μmol/ml, ʻaʻole i ʻoi aku ka nui o ka hemoglobin i hana ʻia e ka hemolysis ma mua o 130g/L, ʻaʻole ʻoi aku ka nui o ke koko lipid ma mua o 65mmol/ml, ʻaʻole ʻoi aku ka nui o ka IgG i loko o ka serum ma mua o 5mg/mL, ʻaʻohe hopena i ka maʻi maʻi dengue a i ʻole ka maʻi chikunguka. ʻO ka maʻi Hepatitis A, ka maʻi Hepatitis B, ka maʻi Hepatitis C, ka maʻi Herpes, ka maʻi maʻi hikina equine encephalitis, Hantavirus, ka maʻi Bunya, ka maʻi maʻi West Nile a me nā mea hoʻohālike genomic serum kanaka i koho ʻia no ka hoʻāʻo cross-reactivity, a ua hōʻike nā hopena ʻaʻohe ʻano ʻokoʻa ma waena o kēia pahu a me nā pathogens i ʻōlelo ʻia ma luna. |
| Nā mea hana pili | ABI 7500 Pūnaehana PCR manawa maoliʻO ABI 7500 ʻO nā Pūnaehana PCR ʻOiaʻiʻo wikiwiki QuantStudio®5 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 Mea Hoʻokele Thermal Cycler manawa maoli BioRad CFX96 Pūnaehana PCR Manawa maoli BioRad CFX Opus 96 Pūnaehana PCR Manawa maoli |
Holo Hana
Koho 1.
QIAamp Viral RNA Mini Kit(52904), Nucleic Acid Extraction a i ʻole Purification Reagent(YDP315-R) na Tiangen Biotech(Beijing) Co.,Ltd.ʻO ka unuhipono e unuhi ʻia e like me nā ʻōlelo aʻoaʻo, a ʻo ka nui o ka unuhi ʻōlelo ʻia he 140 μL a ʻo ka nui elution i ʻōlelo ʻia he 60 μL.
Koho 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Pono e unuhiʻia ka unuhiʻana e like me nā kuhikuhi. ʻO 200 μL ka nui o ka laʻana, a ʻo ka nui elution i ʻōlelo ʻia he 80μL.
