Ka maʻi Zika
Inoa huahana
Pahu ʻIke Waikawa Nukleika HWTS-FE002 no ka maʻi Zika (Fluorescence PCR)
Palapala hōʻoia
CE
ʻAlapia
No ka genus Flaviviridae ka maʻi Zika, he maʻi RNA kaula hoʻokahi me ke anawaena o 40-70 nm. Loaʻa iā ia kahi envelope, aia i loko he 10794 nucleotides, a hoʻopili i 3419 mau waikawa amino. Wahi a ke ʻano genotype, ua māhele ʻia i ke ʻano ʻApelika a me ke ʻano ʻĀkia. ʻO ka maʻi Zika kahi maʻi lele koʻikoʻi i hoʻokumu ʻia e ka maʻi Zika, kahi i hoʻoili nui ʻia ma o ka nahu ʻana o nā makika Aedes aegypti. ʻO nā hiʻohiʻona lapaʻau he kuni nui, ʻulaʻula, arthralgia a i ʻole conjunctivitis, a he mea kakaʻikahi ka make. Wahi a ka World Health Organization, hiki ke pili ka neonatal microcephaly a me ka Guillain-Barre syndrome (Guillain-Barré syndrome) me ka maʻi Zika.
Kanal
| ʻOhana | ʻO ka waikawa nucleic o ka maʻi Zika |
| ROX | Mana Kūloko |
Nā Palena ʻenehana
| Ka Waihona | ≤30 ℃ a pale ʻia mai ka mālamalama |
| Ola papa | 12 mahina |
| ʻAno Laʻana | serum hou |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 500 Kope/mL |
| Kūlana kikoʻī | E hoʻohana i ka pahu hana e ʻike i nā hāpana serum me ka maʻi Zika maikaʻi ʻole, a he maikaʻi ʻole nā hopena. Hōʻike nā hopena hoʻāʻo hoʻopilikia i ka wā ʻaʻole ʻoi aku ka nui o ka bilirubin i loko o ke serum ma mua o 168.2μmol/ml, ʻaʻole ʻoi aku ka nui o ka hemoglobin i hana ʻia e ka hemolysis ma mua o 130g/L, ʻaʻole ʻoi aku ka nui o ka lipid koko ma mua o 65mmol/ml, ʻaʻole ʻoi aku ka nui o ka IgG holoʻokoʻa i loko o ke serum ma mua o 5mg/mL, ʻaʻohe hopena i ka maʻi dengue, ka maʻi Zika a i ʻole ka ʻike ʻana i ka maʻi chikungunya. Ua koho ʻia nā hāpana serum Hepatitis A, Hepatitis B, Hepatitis C, Herpes virus, Eastern equine encephalitis virus, Hantavirus, Bunya virus, West Nile virus a me nā hāpana serum genomic kanaka no ka hoʻāʻo cross-reactivity, a hōʻike nā hopena ʻaʻohe cross reaction ma waena o kēia pahu hana a me nā pathogens i ʻōlelo ʻia ma luna. |
| Nā Mea Hana Pili | Nā ʻōnaehana PCR Manawa Maoli ABI 7500Nā ʻōnaehana PCR Manawa Maoli wikiwiki ABI 7500 QuantStudio®5 mau ʻōnaehana PCR Manawa Maoli Nā ʻōnaehana PCR Manawa Maoli SLAN-96P LightCycler®ʻŌnaehana PCR Manawa Maoli 480 ʻŌnaehana ʻIke PCR Manawa Maoli LineGene 9600 Plus MA-6000 Mea Hoʻopololei Wela Manawa Maoli ʻŌnaehana PCR Manawa Maoli BioRad CFX96 ʻŌnaehana PCR Manawa Maoli BioRad CFX Opus 96 |
Kahe Hana
Koho 1.
QIAamp Viral RNA Mini Kit (52904), Nucleic Acid Extraction a i ʻole Purification Reagent (YDP315-R) na Tiangen Biotech (Beijing) Co.,Ltd.ʻO ka unuhi ʻanapono e unuhi ʻia e like me nā kuhikuhi unuhi, a ʻo ka nui o ka unuhi i ʻōlelo ʻia he 140 μL a ʻo ka nui o ka elution i ʻōlelo ʻia he 60 μL.
Koho 2.
ʻO ka Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) a me ka Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006). Pono e unuhi ʻia ka unuhi ʻana e like me nā kuhikuhi. ʻO ka nui o ka laʻana unuhi he 200 μL, a ʻo ka nui o ka elution i ʻōlelo ʻia he 80μL.
