Zika Virus
Inoa mea kūʻai
HWTS-FE002 Zika Virus Nucleic Acid Detection Kit (Fluorescence PCR)
Palapala
CE
Epidemiology
No ka genus Flaviviridae ka ma'i Zika, he ma'i ma'i RNA i ho'opa'a 'ia me ka anawaena o 40-70nm.He envelop kona, aia 10794 nucleotides, a hoʻopili i 3419 amino acids.Wahi a ka genotype, ua māhele ʻia i ke ʻano ʻApelika a me ke ʻano ʻAsia.ʻO ka maʻi maʻi Zika kahi maʻi maʻi maʻi e hoʻemi ʻia e ka maʻi maʻi Zika, ka mea i lawe nui ʻia ma o ka nahu ʻana o nā makika Aedes aegypti.ʻO nā hiʻohiʻona o ka maʻi he maʻi kuni, ʻūlū, arthralgia a i ʻole conjunctivitis, a ʻaʻohe mea make.Wahi a ka World Health Organization, hiki ke pili i ka microcephaly neonatal a me Guillain-Barre syndrome (Guillain-Barré syndrome) me ka maʻi maʻi Zika.
Kanal
| FAM | Zika virus nucleic acid |
| ROX | Hoomalu Kuloko |
Nā ʻāpana ʻenehana
| Waihona | ≤30 ℃ & pale ʻia mai ka mālamalama |
| Kau-ola | 12 mahina |
| ʻAno Hoʻohālike | serum hou |
| Ct | ≤38 |
| CV | ≤5.0% |
| LoD | 500 ng/μL |
| kikoo | ʻAʻole pili nā hopena hoʻāʻo i loaʻa i kēia pahu e ka hemoglobin (<800g/L), bilirubin (<700μmol/L), a me nā lipids koko/triglycerides (<7mmol/L) i ke koko. |
| Nā mea hana pili | ABI 7500 Pūnaehana PCR manawa maoli ʻO ABI 7500 ʻO nā Pūnaehana PCR ʻOiaʻiʻo wikiwiki QuantStudio®5 Pūnaehana PCR manawa maoli SLAN-96P Pūnaehana PCR Manawa maoli LightCycler®480 Pūnaehana PCR manawa maoli LineGene 9600 Plus Pūnaehana ʻike PCR manawa maoli MA-6000 ʻOiaʻiʻo ka nui o ka wela wela ʻO BioRad CFX96 Pūnaehana PCR manawa maoli, BioRad CFX Opus 96 Pūnaehana PCR manawa maoli. |
Holo Hana
Koho 1.
QIAamp Viral RNA Mini Kit(52904), Nucleic Acid Extraction a i ʻole Purification Reagent(YDP315-R) na Tiangen Biotech(Beijing) Co.,Ltd.ʻO ka unuhiPono e unuhi ʻia e like me nā ʻōlelo aʻoaʻo, a ʻo ka nui o ka unuhi ʻōlelo ʻia he 140 μL a ʻo ka nui elution i ʻōlelo ʻia he 60 μL.
Koho 2.
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) a me Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006).Pono e unuhiʻia ka unuhiʻana e like me nā kuhikuhi.ʻO 200 μL ka nui o ka laʻana, a ʻo ka nui o ka elution i ʻōlelo ʻia he 80μL.
